Validation of human skin models for skin corrosivity tests in Japan

• Kojima Hajime

  Japanese Center for the Validation of Alternative Methods (JaCVAM), National Institute of Health Sciences (NIHS) Res.Lab., Nippon Menard Cosmetic Co., Ltd.

• Ando Tomoko

  Div. Cellular and Molecular Toxicol. NIHS

• Inagaki Katsuhiro

  Res. & Develop., Div., Product Safety & Pharmaceutical Research Unit, Nihon Nohyaku Co., Ltd.

• Ohhira Mahito

  Toxicol. Res. Dep.,ODAWARA Res. Center, Nippon Soda Co.,Ltd.

• Kosaka Tadashi

  Toxicol. Div. The Inst. Environ. Toxicol.

• Nakamura Yosuke

  Environ. Health Sci. Lab., Sumitomo Chemical Co., Ltd.

• Torishima Hisashi

  Bio-Medical Dep., Kurabo Industries Ltd.

• Morikawa Noriyuki

  Div. R&D, Gunze Ltd.

• Kanno Jun

  Div. Cellular and Molecular Toxicol. NIHS

• Kuboki Mami

  Toxicol. Res. Dep.,ODAWARA Res. Center, Nippon Soda Co.,Ltd.

• Genno Michiru

  Bio-Medical Dep., Kurabo Industries Ltd.

• Nokata Masaru

  Res. & Develop., Div., Product Safety & Pharmaceutical Research Unit, Nihon Nohyaku Co., Ltd.

• Harada Takanori

  Toxicol. Div. The Inst. Environ. Toxicol.

• Morimoto Takashi

  Environ. Health Sci. Lab., Sumitomo Chemical Co., Ltd.

• Yoshimura Isao

  Fac. Eng. Tokyo Univ. Science

• Ohno Yasuo

  NIHS

Abstract

As shown in OECD test guidelines 430 and 431, the human skin epidermal assay and Transcutaneous Electrical Resistance Test (TER) were validated and peer reviewed as an alternative method to corrosivity testing; however, these methods have not been used widely in Japan. The problems related to techniques and evaluation are not clear. Therefore, we performed a validation study of EPI-200 (EpiDerm^TM), a 3-dimensional cultured epidermal model and Vitrolife-Skin^TM, a 3-dimensional cultured skin model made in Japan as a catch-up validation trial of alternatives for skin corrosivity testing using 13 chemicals including a positive control: 10% potassium hydroxide solution in Japan. From the obtained data, we identified the potential of utilizing these models to evaluate the corrosivity of a chemical.

Journal

• Alternatives to Animal Testing and Experimentation

  Alternatives to Animal Testing and Experimentation 13 (1), 36-44, 2008

  Japanese Society for Alternative to Animal Experiments

Keywords

• skin corrosivity
• cultured epidermal model
• cultured skin model
• validation

Details

• CRID: 1390282680211367424
• NII Article ID: 130004556181
• NII Article ID: 10021095170
• NII Book ID: AA11161787
• DOI: 10.11232/aatex.13.36
• ISSN: 21854726
• ISSN: 13440411
• Web Site: https://search.jamas.or.jp/link/ui/2009059157
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed