Alizarin Complexone Stain for Histochemical Localization of Lanthanum

• Miyagawa Makoto

  Central Experimental Animal Center, School of Medicine, Teikyo University

Bibliographic Information

Other Title

• Alizarin ComplexoneによるLaの組織化学的染色法
• Alizarin Complexone ニ ヨル La ノ ソシキ カガクテキ センショクホウ

Abstract

In recent years, lanthanum(La)compounds have been increasingly used in the optical and electronic industries. Such industrial sources of La pose potential environmental problems, and it is therefore necessary to investigate the effects of La on humans and animals. A simple technique involving staining of La at the light microscopic level with alizarin complexone has been developed. This method is based on the formation of insoluble alizarin complexone-La-F in response to NaF treatment. The reactive material is reddish-purple in color and shows on absorption maximum at 530 nm. Application of this method to paraffin-embedded liver sections from rats treated with LaCl₃ allowed for the selective staining of La at the light microscopic level. Thus, this method can be used for pathologic diagnosis in paraffin-embedded sections.

Journal

• Biomedical Research on Trace Elements

  Biomedical Research on Trace Elements 18 (4), 375-379, 2007

  Japan Society for Biomedical Research on Trace Elements

Keywords

• lanthanum
• alizarin complexone(ALC)
• rats
• paraffin section
• histochemical stain

Details

• CRID: 1390001204367462912
• NII Article ID: 10021097724
• NII Book ID: AN10423256
• DOI: 10.11299/brte.18.375
• ISSN: 18801404
• ISSN: 0916717X
• NDL BIB ID: 9412491
• Web Site: http://id.ndl.go.jp/bib/9412491
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9412491
• Web Site: https://search.jamas.or.jp/link/ui/2008203886
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed