The effects of dibutyltin (DBT) dichloride on the viability and the productions and tumor necrosis factor α and interleukin-12 in murine macrophage cell line, J774.1
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- Tsunoda Masashi
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine
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- Yoshida Tamae
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine Kitasato Medical Service, Co.
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- Tsuji Masayoshi
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine Mejiro University College
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- Zhang Ying
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine Fukushima Rehabilitation Academy
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- Sugaya Chiemi
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine
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- Inoue Yoko
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine
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- Miki Takeo
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine
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- Kudo Yuichiro
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine
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- Satoh Toshihiko
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine
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- Aizawa Yoshiharu
- Department of Preventive Medicine and Public Health,Kitasato University School of Medicine
書誌事項
- タイトル別名
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- The Effects of Dibutyltin(DBT)Dichloride on the Viability and the Productions of Tumor Necrosis Factor α and Interleukin-12 in Murine Macrophage Cell Line, J774.1.
- The effects of dibutyltin DBT dichloride on the viability and the productions and tumor necrosis factor a and interleukin 12 in murine macrophage cell line J774 1
この論文をさがす
抄録
Immunotoxicity is one of toxic effects of dibutyltin(DBT)compounds. In our previous study, the inhibitory effects of DBT on the productions of tumor necrosis a(TNFα)and interleukin-1β in a murine macrophage cell line, J774.1, were demonstrated. In this study, the effects of DBT dichloride from 0.125 µM to 2.0 µM on the cell viability and productions of cytokines including TNFα, IL-10 and IL-12p40 were investigated. J774.1 cells were exposed to DBT dichloride at 0, 0.125, 0.25, 0.5, 1.0, 1.5 or 2.0 µM. After 18 hours, lipopolysaccharide was added to each well. Total RNA was extracted from the cells after an additional 6 hours of incubation. Real-time PCR was used to analyze the mRNA expression for TNFα, IL-10, IL-12p40 and glycelaldehyde-3-phosphate dehydrogenase(GAPDH)in J774.1 cells. The cell viabilities were determined and the supernatants were sampled after additional 24 hours of incubation. The concentrations of TNFα, IL-10 and IL-12p40 in the supernatant were determined by ELISA. The mean cell viabilities in the groups exposed to DBT at 0.5 µM and over were significantly lower than that of the control. The mean mRNA expression of IL-12p40 in the 0.25 µM group was significantly higher than that in the control group. The mean concentrations of TNFα in the supernatant in the 1.0, 1.5 and 2.0 µM groups were significantly lower than that of the control, and that in 0.125, 0.25 and 0.5 µM groups were significantly higher. The mean concentration of IL-12p40 in the 0.25 µM group was significantly higher than that of the control and that in the 1.0, 1.5 and 2.0 µM groups were significantly lower. There were no significant differences in mRNA expression and protein level for IL-10. DBT is toxic to macrophages, and the inhibition of the production of cytokines by DBT does not occur for all cytokines.
収録刊行物
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- Biomedical Research on Trace Elements
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Biomedical Research on Trace Elements 19 (1), 67-71, 2008
日本微量元素学会
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詳細情報 詳細情報について
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- CRID
- 1390282679344494208
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- NII論文ID
- 130004456852
- 10021098042
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- NII書誌ID
- AN10423256
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- COI
- 1:CAS:528:DC%2BD1cXosVagsrk%3D
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- ISSN
- 18801404
- 0916717X
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- NDL書誌ID
- 9542085
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
- Crossref
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- 抄録ライセンスフラグ
- 使用不可