Functional Analysis of Two Homologous Mouse Selenophosphate Synthetases

• Abe Katsumasa

  Institute for Chemical Research, Kyoto University

• Mihara Hisaaki

  Institute for Chemical Research, Kyoto University

• Nishijima Yoshihito

  Institute for Chemical Research, Kyoto University

• Kurokawa Suguru

  Institute for Chemical Research, Kyoto University

• Esaki Nobuyoshi

  Institute for Chemical Research, Kyoto University

抄録

Selenophosphate, a key intermediate in selenium metabolism, is synthesized from selenide and ATP by selenophosphate synthetase(SPS). Mammals have two SPS isozymes known as SPS1 and SPS2. Mouse SPS2 contains a catalytically important selenocysteine residue at position 63 of the polypeptide chain. In contrast, SPS1 has a Thr-29 in the position that corresponds to Sec-63 of SPS2. SPS1 and Sec63Cys mutant SPS2 were purified to homogeneity and characterized. SPS2(Sec63Cys)catalyzed the selenide-dependent synthesis of selenophosphate, but SPS1 showed no activity. However, the Thr29Cys mutant of SPS1 exhibited SPS activity. These findings raise the intriguing possibility that SPS1 may exert its catalytic function in the presence of thiols in the vicinity of position 29.

収録刊行物

• Biomedical Research on Trace Elements

  Biomedical Research on Trace Elements 19 (1), 76-79, 2008

  日本微量元素学会

キーワード

• selenium
• selenophosphate
• selenoprotein
• selenophosphate synthetase
• mouse

詳細情報

• CRID: 1390282679344510592
• NII論文ID: 130004456854
• NII論文ID: 10021098062
• NII書誌ID: AN10423256
• DOI: 10.11299/brte.19.76
• ISSN: 18801404
• ISSN: 0916717X
• NDL書誌ID: 9542141
• Web Site: http://id.ndl.go.jp/bib/9542141
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9542141
• Web Site: https://search.jamas.or.jp/link/ui/2008358484
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可