Evaluation of Cell-free Protein Synthesis Using PDMS-based Microreactor Arrays

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著者

    • NOJIMA Takahiko
    • Center for International Research on MicroMechatronics (CIRMM), Institute of Industrial Science, The University of Tokyo
    • BABA Yoshinobu
    • Department of Applied Chemistry, Graduate School of Engineering, MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
    • FUJII Teruo
    • Center for International Research on MicroMechatronics (CIRMM), Institute of Industrial Science, The University of Tokyo

抄録

A living cell has numerous proteins, only a few thousand of which have been identified to date. Cell-free protein synthesis is a useful and promising technique to discover and produce various proteins that might be beneficial for biotechnological, pharmaceutical, and medical applications. For this study, we evaluated the performance and the general applicability of our previously developed microreactor array chip to cell-free protein synthesis by comparisons with a commercially available system. The microreactor array chip comprises a temperature control chip made of glass and a disposable reaction chamber chip made of polydimethylsiloxane (PDMS). For evaluation of the microreactor array chip, rat adipose-type fatty acid binding protein, glyceraldehyde-3-phosphate dehydrogenase, cyclophilin, and firefly luciferase were synthesized from their respective DNA templates using a cell-free extract prepared from <i>Escherichia coli</i>. All these proteins were synthesized in the microreactor array chip, and their respective amounts and yields were investigated quantitatively.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 24(2), 243-246, 2008-02-10 

    The Japan Society for Analytical Chemistry

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各種コード

  • NII論文ID(NAID)
    10021098477
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    9362912
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  IR  J-STAGE 
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