Phospholipid-linked Coumarin: A Fluorescent Probe for Sensing Hydroxyl Radicals in Lipid Membranes

• SOH Nobuaki

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• MAKIHARA Koji

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• ARIYOSHI Tomoyuki

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• SETO Daisuke

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• MAKI Tomoharu

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• NAKAJIMA Hizuru

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• NAKANO Koji

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• IMATO Toshihiko

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

抄録

A fluorescent probe, DPPEC (1,2-dipalmitoylglycerophosphorylethanolamine labeled with coumarin) was developed for detecting hydroxyl radical (·OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of ·OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes rapidly reacted with ·OH and increased the fluorescence intensity, depending on the concentration of ·OH. The increase in the fluorescence intensity induced by ·OH was effectively suppressed by the addition of DMSO. The probe exhibited a higher fluorescence response to ·OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect ·OH efficiently.

収録刊行物

• Analytical Sciences

  Analytical Sciences 24 (2), 293-296, 2008

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390282679235136000
• NII論文ID: 130004441618
• NII論文ID: 10021098715
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.24.293
• COI: 1:CAS:528:DC%2BD1cXit1Onsrg%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 9362977
• PubMed: 18270426
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9362977
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.24.293.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.24.293/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/24/2/24_2_293/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可