Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

DOI PDF Web Site Web Site Web Site 参考文献51件
  • HIRATSUKA Atsunori
    Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), c/o Katayanagi Advanced Research Laboratories, Tokyo University of Technology
  • FUJISAWA Kohta
    Department of Electronic Engineering, Shibaura Institute of Technology
  • MUGURUMA Hitoshi
    Department of Electronic Engineering, Shibaura Institute of Technology

この論文をさがす

抄録

A novel design is described for an amperometric biosensor based on NAD(P)-dependent glucose dehydrogenase (GDH) combined with a plasma-polymerized thin film (PPF). The GDH is sandwiched between several nanometer thick acetonitrile PPFs on a sputtered gold electrode (PPF/GDH/PPF/Au). The lower PPF layer plays the role as an interface between enzyme and electrode because it is extremely thin, adheres well to the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. The upper PPF layer (overcoating) was directly deposited on immobilized GDH. The optimized amperometric biosensor characteristics covered 2.5 - 26 mM glucose concentration at +0.6 V of applied potential; the least-squares slope was 320 nA mM-1 cm-2 and the correlation coefficient was 0.990. Unlike conventional wet-chemical processes that are incompatible with mass production techniques, this dry-chemistry procedure has great potential for enabling high-throughput production of bioelectronic devices.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 24 (4), 483-486, 2008

    社団法人 日本分析化学会

参考文献 (51)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ