Spectrophotometric Determination of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride by Flow Injection Analysis

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著者

    • Seno Kunihiko SENO Kunihiko
    • Department of Chemistry, Faculty of Science, Graduate School of Natural Sciences and Technology, Okayama University
    • MOTOMIZU Shoji
    • Department of Chemistry, Faculty of Science, Graduate School of Natural Sciences and Technology, Okayama University

抄録

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium. A simple and fast detection system was developed using the reaction with pyridine and ethylenediamine in acidic aqueous solution and spectrophotometric flow injection analysis. The absorbances were measured at 400 nm and the reaction was accelerated at 40°C. The calibration graph showed good linearity from 0 to 10% of EDC·HCl solutions: the regression equation was <i>y</i> = 3.15 × 10<sup>4</sup><i>x</i> (<i>y</i>, peak area; <i>x</i>, % concentration of EDC·HCl). The RSD was under 1.0%. Sample throughput was 15 h<sup>-1</sup>. This method was applied to monitoring the EDC·HCl concentration that remained after the anhydration of phthalic acid in water, esterification of acetic acid in methanol or dehydration condensation of malonic acid and ethylenediamine in water.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 24(4), 505-508, 2008-04-10 

    The Japan Society for Analytical Chemistry

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各種コード

  • NII論文ID(NAID)
    10021099678
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    9452293
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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