Comparison of Enzymatic Recycling Electrodes for Measuring Aminophenol: Development of a Highly Sensitive Natriuretic Peptide Assay System
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- MIE Yasuhiro
- Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)
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- KOWATA Keiko
- Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)
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- HIRANO Yu
- Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)
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- NIWA Osamu
- Tsukuba Center, National Institute of Advanced Industrial Science and Technology (AIST)
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- MIZUTANI Fumio
- Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)
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Abstract
Several redox enzymes were examined for enzymatic/electrochemical-recycling systems in order to measure p-aminophenol (PAP) with high sensitivity. Glucose oxidase (GOD) and diaphorase (DI) worked well as catalysts for recycling electrode systems: these enzymes effectively reduced p-iminoquinone (PIQ), the electrochemically-oxidized form of PAP, and caused an enhancement in the electrochemical signals (anodic currents in the voltammogram and amperogram) by ∼ 100 fold. The lower detection limits for PAP were estimated to be 50 nM with the GOD system and 2 nM with the DI system. We combined the enzymatic-recycling electrode using DI with an enzyme immunoassay system to measure atrial natriuretic peptide (ANP), an important marker peptide hormone involved in heart diseases. ANPs from serum samples at ppt-levels were determined appropriately using the present assay system.
Journal
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- Analytical Sciences
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Analytical Sciences 24 (5), 577-582, 2008
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679233418880
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- NII Article ID
- 130004441646
- 10021099931
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 9483008
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed