Protein Expression of Saccharomyces cerevisiae in Response to Uranium Exposure

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著者

    • SAKAMOTO F.
    • Advanced Science Research Center, Japan Atomic Energy Agency
    • NANKAWA T.
    • Advanced Science Research Center, Japan Atomic Energy Agency
    • KOZAI N.
    • Advanced Science Research Center, Japan Atomic Energy Agency
    • FUJII T.
    • Environmental Research Division, National Research Institute of Brewing
    • IEFUJI H.
    • Environmental Research Division, National Research Institute of Brewing
    • FRANCIS A. J.
    • Environmental Sciences Department, Brookhaven National Laboratory
    • OHNUKI T.
    • Advanced Science Research Center, Japan Atomic Energy Agency

抄録

Protein expression of Saccharomyces cerevisiae grown in the medium containing <SUP>238</SUP>U (VI) and <SUP>233</SUP>U (VI) was examined by two-dimensional gel electrophoresis. Saccharomyces cerevisiae of BY4743 was grown in yeast nitrogen base medium containing glucose and glycerol 2-phosphate and <SUP>238</SUP>U of 0, 2.0, and 5.0×10<SUP>-4</SUP> M or <SUP>233</SUP>U of 2.5×10<SUP>-6</SUP> M (radioactivity was higher by 350 times than 2.0×10<SUP>-4</SUP> M <SUP>238</SUP>U) and 5.0×10<SUP>-6</SUP> M for 112 h at 30°C. The growth of Saccharomyces cerevisiae was monitored by measuring OD<SUB>600</SUB> at 112 h after the inoculation. Uranium concentrations in the media also were measured by radiometry using a liquid scintillation counter. The growths of the yeast grown in the above media were in the following order: control > 2.5×10<SUP>-6</SUP> M <SUP>233</SUP>U > 2.0×10<SUP>-4</SUP> M <SUP>238</SUP>U > 5.0×10<SUP>-6</SUP> M <SUP>233</SUP>U > 5.0×10<SUP>-4</SUP> M <SUP>238</SUP>U. This result indicated that not only radiological but also chemical effect of U reduced the growth of the yeast. The concentrations of U in the medium containing <SUP>238</SUP>U or <SUP>233</SUP>U decreased, suggesting U accumulation by the yeast cells. The 2-D gel electrophoresis analysis showed the appearance of several spots after exposure to <SUP>238</SUP>U or to <SUP>233</SUP>U but not in the control containing no uranium. These results show that the yeast cells exposed to U express several specific proteins.

収録刊行物

  • Journal of Nuclear and Radiochemical Sciences  

    Journal of Nuclear and Radiochemical Sciences 8(2), 133-136, 2007-12-01 

    The Japan Society of Nuclear and Radiochemical Sciences

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各種コード

  • NII論文ID(NAID)
    10021102172
  • NII書誌ID(NCID)
    AA11948402
  • 本文言語コード
    ENG
  • 資料種別
    REV
  • ISSN
    13454749
  • データ提供元
    CJP書誌  J-STAGE 
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