Protein Expression of Saccharomyces cerevisiae in Response to Uranium Exposure

• Sakamoto F.

  Advanced Science Research Center, Japan Atomic Energy Agency

• Nankawa T.

  Advanced Science Research Center, Japan Atomic Energy Agency

• Kozai N.

  Advanced Science Research Center, Japan Atomic Energy Agency

• Fujii T.

  Environmental Research Division, National Research Institute of Brewing

• Iefuji H.

  Environmental Research Division, National Research Institute of Brewing

• Francis A. J.

  Environmental Sciences Department, Brookhaven National Laboratory

• Ohnuki T.

  Advanced Science Research Center, Japan Atomic Energy Agency EcoTopia Science Institute, Nagoya University

Abstract

Protein expression of Saccharomyces cerevisiae grown in the medium containing ²³⁸U (VI) and ²³³U (VI) was examined by two-dimensional gel electrophoresis. Saccharomyces cerevisiae of BY4743 was grown in yeast nitrogen base medium containing glucose and glycerol 2-phosphate and ²³⁸U of 0, 2.0, and 5.0×10^-4 M or ²³³U of 2.5×10^-6 M (radioactivity was higher by 350 times than 2.0×10^-4 M ²³⁸U) and 5.0×10^-6 M for 112 h at 30°C. The growth of Saccharomyces cerevisiae was monitored by measuring OD₆₀₀ at 112 h after the inoculation. Uranium concentrations in the media also were measured by radiometry using a liquid scintillation counter. The growths of the yeast grown in the above media were in the following order: control > 2.5×10^-6 M ²³³U > 2.0×10^-4 M ²³⁸U > 5.0×10^-6 M ²³³U > 5.0×10^-4 M ²³⁸U. This result indicated that not only radiological but also chemical effect of U reduced the growth of the yeast. The concentrations of U in the medium containing ²³⁸U or ²³³U decreased, suggesting U accumulation by the yeast cells. The 2-D gel electrophoresis analysis showed the appearance of several spots after exposure to ²³⁸U or to ²³³U but not in the control containing no uranium. These results show that the yeast cells exposed to U express several specific proteins.

Journal

• Journal of Nuclear and Radiochemical Sciences

  Journal of Nuclear and Radiochemical Sciences 8 (2), 133-136, 2007

  The Japan Society of Nuclear and Radiochemical Sciences

Details

• CRID: 1390282679458312448
• NII Article ID: 10021102172
• NII Book ID: AA11948402
• DOI: 10.14494/jnrs2000.8.133
• COI: 1:CAS:528:DC%2BD2sXhsVCgtbvN
• ISSN: 18835813
• ISSN: 13454749
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed