Micro-Flow System Comprised of a Fused-Silica Capillary and Chemiluminescence Detection that Works under Laminar Flow Conditions

この論文にアクセスする

この論文をさがす

著者

抄録

A micro-flow system comprised of a fused-silica capillary and chemiluminescence detection was developed, in which a wide-bore hydrodynamic chromatography mode was first introduced into the system. The micro-flow system was run with a gravity method or pump flow method for feeding a carrier solution into the capillary. Oxalate–hydrogen peroxide chemiluminescence reaction was adopted as a detection technique. Fluorescent compound (fluorescein isothiocyanate; FITC) and FluoSphere fluorescent polymer particles with particle diameters of 0.02 μm and 1.0 μm were used as model samples in the present system. FITC and FluoSpheres (0.02 μm) with diffusion indexes greater than ca. 1 were delivered into the capillary with average linear velocities under laminar flow conditions, which led to a Gaussian peak on the chemiluminescence profile. On the other hand, FluoSpheres (1.0 μm) with a diffusion index of ca. 0.1 were delivered with the approximately maximum linear velocity and formed a non-Gaussian peak. The mixtures, FITC and FluoSpheres (1.0 μm) as well as FluoSpheres (0.02 μm) and FluoSpheres (1.0 μm), reflected the individual diffusion indexes, indicating two main peaks on the chemiluminescence profiles. An unexpected small Gaussian peak observed with the FluoSpheres (1.0 μm) sample was also observed.

収録刊行物

  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 41(2), 130-137, 2008-02-01 

    The Society of Chemical Engineers, Japan

参考文献:  13件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  1件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021109758
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • NDL 記事登録ID
    9384085
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ