Enzymatic Production of High Concentration Glucose-1-Phosphate

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Glucose-1-phosphate (G-1-P) is a biological product which, along with its derivatives, has various physiological applications. In order to develop an efficient industrial process for G-1-P, enzymatic production of high concentration G-1-P was attempted by using phosphorylase purified from potatoes as an enzyme, several kinds of linear and branched dextrin differing in chain length and a mixture of KH<SUB>2</SUB>PO<SUB>4</SUB> and K<SUB>2</SUB>HPO<SUB>4</SUB> (Pi) as substrates. Since the maltotetraose is not active as a substrate for production of G-1-P, effective glucan concentration was defined on a basis of moles of glucose (<I>C</I><SUB>eG</SUB>) excluding a segment of maltotetraose from dextrin. Furthermore, since the reaction was a complicated reversible reaction involving many kinds of glucan differing in chain length, the inhibitory effect of a high concentration of substrate for the enzyme, an apparent equilibrium constant <I>K</I><SUB>app</SUB> was defined. <I>K</I><SUB>app</SUB> was expressed as a function of initial concentration of glucan (<I>C</I><SUB>eG,0</SUB>) and phosphate (<I>C</I><SUB>P,0</SUB>). The concentration of G-1-P finally obtained at the equilibrium (<I>C</I><SUB>G1P,eq</SUB>) could be easily calculated from <I>K</I><SUB>app</SUB> regardless of chain length and branch structures of glucan. As a result, a diagram expressing <I>C</I><SUB>G1P,eq</SUB> as a function of <I>C</I><SUB>eG,0</SUB> and <I>C</I><SUB>P,0</SUB> was proposed. A high concentration of G-1-P, namely 230 mM G-1-P was experimentally obtained when <I>C</I><SUB>eG,0</SUB> and <I>C</I><SUB>P,0</SUB> were 516 and 3000 mM, respectively, and the enzymatic activity was 100 U·mL<SUP>–1</SUP>.

収録刊行物

  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 41(4), 306-311, 2008-04-01 

    The Society of Chemical Engineers, Japan

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各種コード

  • NII論文ID(NAID)
    10021110124
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • NDL 記事登録ID
    9463055
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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