Enzymatic Production of High Concentration Glucose-1-Phosphate
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- Sakata Masaru
- Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University Processing Development Research Laboratories, Kao Corp.
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- Kawai Takashi
- Processing Development Research Laboratories, Kao Corp.
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- Kayane Shigeto
- Research & Development Division, Kao Corp.
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- Ooshima Hiroshi
- Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
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Abstract
Glucose-1-phosphate (G-1-P) is a biological product which, along with its derivatives, has various physiological applications. In order to develop an efficient industrial process for G-1-P, enzymatic production of high concentration G-1-P was attempted by using phosphorylase purified from potatoes as an enzyme, several kinds of linear and branched dextrin differing in chain length and a mixture of KH2PO4 and K2HPO4 (Pi) as substrates. Since the maltotetraose is not active as a substrate for production of G-1-P, effective glucan concentration was defined on a basis of moles of glucose (CeG) excluding a segment of maltotetraose from dextrin. Furthermore, since the reaction was a complicated reversible reaction involving many kinds of glucan differing in chain length, the inhibitory effect of a high concentration of substrate for the enzyme, an apparent equilibrium constant Kapp was defined. Kapp was expressed as a function of initial concentration of glucan (CeG,0) and phosphate (CP,0). The concentration of G-1-P finally obtained at the equilibrium (CG1P,eq) could be easily calculated from Kapp regardless of chain length and branch structures of glucan. As a result, a diagram expressing CG1P,eq as a function of CeG,0 and CP,0 was proposed. A high concentration of G-1-P, namely 230 mM G-1-P was experimentally obtained when CeG,0 and CP,0 were 516 and 3000 mM, respectively, and the enzymatic activity was 100 U·mL–1.
Journal
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- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
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JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 41 (4), 306-311, 2008
The Society of Chemical Engineers, Japan
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Details 詳細情報について
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- CRID
- 1390282679545104000
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- NII Article ID
- 10021110124
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- NII Book ID
- AA00709658
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- ISSN
- 18811299
- 00219592
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- NDL BIB ID
- 9463055
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed