Effect of Nucleotide Sequences of Polypeptide Linkers on Production of Soluble Single-Chain Fv Antibodies

DOI Web Site Web Site 被引用文献1件 参考文献18件
  • Kumada Yoichi
    Department of Chemistry and Materials Technology, Kyoto Institute of Technology
  • Kawasaki Tomomi
    Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University
  • Sakan Yoshinobu
    Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University
  • Kikuchi Yasufumi
    Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University
  • Katoh Shigeo
    Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University

この論文をさがす

抄録

Effects of nucleotide sequences of polypeptide linkers on production of soluble single-chain Fv antibodies in E. coli were studied using scFv genes, in which genes of VH and VL domains were genetically linked with 4 types of linker genes with or without rare codons, namely genes of scFv (G4S)3, scFv (G4S)3R, scFv No. 10 and scFv No. 10NR. The transformants expressing scFvs having linkers with 3–4 rare codons (scFv (G4S)3R and scFv No. 10) could grow to higher cell concentrations under induction condition, while growth of the cells expressing scFvs having linkers without rare codon (scFv (G4S)3 and scFv No. 10NR) stopped after 3 h. Productivity levels of soluble scFv by cells expressing scFv (G4S)3R and scFv No. 10 were higher than those of scFv (G4S)3 and scFv No. 10NR. The specific productivity levels of inclusion bodies for scFv (G4S)3R and scFv No. 10 decreased in comparison with those for scFv (G4S)3 and scFv No. 10NR. This might show that decrease in the translation rate at the region coding polypeptide linkers with rare codons has a favorable effect for the subsequent folding process of scFv molecules. Furthermore, from a SPR analysis using an antigen-immobilized sensor chip, scFv (G4S)3R showed the same binding affinity as the original scFv (G4S)3. Thus, nucleotide sequences of polypeptide linkers are important to increase productivity of scFv expressed in soluble fraction. The nucleotide sequence of the flexible linker (G4S)3R used in this study may be useful for efficient production of various scFvs without loss of the binding affinity of target scFv.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (18)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ