Construction of Manganese Porphyrin Modified Hemoglobin Complex and Its Antioxidant Activities

• MURATA Hidenori

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science Research Institute for Science and Technology, Tokyo University of Science

• ITO Yuji

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science

• SHIMPO Tomoyuki

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science

• OYAIZU Kenichi

  Research Institute for Science and Technology, Tokyo University of Science

• YUASA Makoto

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science Research Institute for Science and Technology, Tokyo University of Science

Bibliographic Information

Other Title

• マンガンポルフィリン／化学修飾ヘモグロビン複合体の構築とその抗酸化作用
• マンガン ポルフィリン カガク シュウショク ヘモグロビン フクゴウタイ ノ コウチク ト ソノ コウサンカ サヨウ

Abstract

Manganese 5,10,15,20-tetrakis(N-methyl-2-yl) porphyrin (MnT2MePyP) was introduced to hemoglobin to use it as a drug carrier. Reconstituted hemoglobin was reacted with pyridoxal-5-phosphate and borohydride to yield pyridoxalated reconstituted hemoglobin (PLPrHb). The reaction of PLPrHb with activated polyethyleneglycol gave pegylated reconstituted hemoglobin (PEGrHb). PEGrHb was polymerized using glutaraldehyde to yield cross-linked pegylated reconstituted hemoglobin (Cross-link PEGrHb). The number average moleculer weight of Cross-link PEGrHb was estimated to be 1.0×10⁵ by GPC. The modification of hemoglobin was checked by using SDS-PAGE. Stopped flow analysis showed a high SOD activity of Cross-link PEGrHb. A pharmacokinetic study of ¹²⁵I-labelled Cross-link PEGrHb that was infused into mice revealed a long-term blood circulation. The novel Manganese porphyrin modified hemoglobin complex was found to remain long-term in the blood circulation without being removed by excretion. These results showed that this complex can be used as a high performance antioxidant.<br>

Journal

• KOBUNSHI RONBUNSHU

  KOBUNSHI RONBUNSHU 65 (4), 277-282, 2008

  The Society of Polymer Science, Japan

Keywords

• Antioxidant
• Manganese Porphyrin
• Hemoglobin
• Cross-link

Details

• CRID: 1390282681501341824
• NII Article ID: 10021120303
• NII Book ID: AN00085011
• DOI: 10.1295/koron.65.277
• ISSN: 18815685
• ISSN: 03862186
• NDL BIB ID: 9492229
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9492229
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed