酵素エッチングを施した羊毛繊維断面の走査型電子顕微鏡観察 SEM Images of Wool Fiber Cross Sections Etched by Means of Protease Digestion

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抄録

To identify some fine histological features of wool fiber cross-sections by scanning electron microscopy (SEM), the preparation method by the differential dissolution of section with a proteolytic enzyme was investigated. Wool fibers were embedded in epoxy resin and were sectioned into about 25 μm thickness. The resultant sections were mounted on the SEM sample stage and were surface etched by enzyme (Savinase: Nobozymes Japan industry) solution followed by SEM observation. By using this method for preparing specimens, this differential digestion of components revealed the relatively distinct histological structure of the fiber in the SEM images with good resolution, where the cystine-poor endocuticle, cell membranes, nuclear remnants, and intermacrofibrillar material of the intact wool cross-sections were partially dissolved away. For the wool fiber oxidized under aqueous acidic conditions with Caro's salt, especially, the macrofibrils were observed in an electron scattering zone (concave circular structure) at the center of each one over the orthocortex, but not over the paracortex. Because of this differentiation, it was possible to distinctly discriminate between the ortho- and pracortex cell types of the fiber. In addition, this method will undoubtedly be valuable for assessing the extent of damage in fiber resulting from various physical and chemical treatments.

収録刊行物

  • 繊維学会誌

    繊維学会誌 64(5), 118-124, 2008-05-10

    The Society of Fiber Science and Technology, Japan

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各種コード

  • NII論文ID(NAID)
    10021127032
  • NII書誌ID(NCID)
    AN00131651
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00379875
  • NDL 記事登録ID
    9518290
  • NDL 雑誌分類
    ZP33(科学技術--繊維工学・工業)
  • NDL 請求記号
    Z17-189
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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