SEM Images of Wool Fiber Cross Sections Etched by Means of Protease Digestion

• Okada Michiko

  Kyoto Institute of Technology, Hashikami-cho, Matsugasaki Sakyo-ku, Kyoto 606-0951, Japan

• Kimura Yoshiharu

  Kyoto Institute of Technology, Hashikami-cho, Matsugasaki Sakyo-ku, Kyoto 606-0951, Japan

• Maekawa Masako

  Nara Women's University, Kita-uoyahigashi, Nara 603-8506, Japan

• Joko Kyohei

  Kyoto Women's University, Kita-hiyoshi, Imakumano, Higashiyama-ku, Kyoto 605-8501, Japan

Bibliographic Information

Other Title

• 酵素エッチングを施した羊毛繊維断面の走査型電子顕微鏡観察
• コウソ エッチング オ ホドコシタ ヨウモウ センイ ダンメン ノ ソウサガタ デンシ ケンビキョウ カンサツ

Abstract

To identify some fine histological features of wool fiber cross-sections by scanning electron microscopy (SEM), the preparation method by the differential dissolution of section with a proteolytic enzyme was investigated. Wool fibers were embedded in epoxy resin and were sectioned into about 25 μm thickness. The resultant sections were mounted on the SEM sample stage and were surface etched by enzyme (Savinase: Nobozymes Japan industry) solution followed by SEM observation. By using this method for preparing specimens, this differential digestion of components revealed the relatively distinct histological structure of the fiber in the SEM images with good resolution, where the cystine-poor endocuticle, cell membranes, nuclear remnants, and intermacrofibrillar material of the intact wool cross-sections were partially dissolved away. For the wool fiber oxidized under aqueous acidic conditions with Caro's salt, especially, the macrofibrils were observed in an electron scattering zone (concave circular structure) at the center of each one over the orthocortex, but not over the paracortex. Because of this differentiation, it was possible to distinctly discriminate between the ortho- and pracortex cell types of the fiber. In addition, this method will undoubtedly be valuable for assessing the extent of damage in fiber resulting from various physical and chemical treatments.

Journal

• Sen'i Gakkaishi

  Sen'i Gakkaishi 64 (5), 118-124, 2008

  The Society of Fiber Science and Technology, Japan

Keywords

• General Medicine

Details

• CRID: 1390282679842379392
• NII Article ID: 10021127032
• NII Book ID: AN00131651
• DOI: 10.2115/fiber.64.118
• ISSN: 18842259
• ISSN: 00379875
• NDL BIB ID: 9518290
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9518290
• Web Site: http://www.jstage.jst.go.jp/article/fiber/64/5/64_5_118/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed