分子拡散培養法を用いた培養心筋細胞の成長過程における自律拍動リズムの時間変化 Developmental Changes in Spontaneous Beating Rhythm of Cardiac Myocytes in vitro Cultured with Molecular Diffusion Culture Method

この論文にアクセスする

この論文をさがす

著者

抄録

To understand the detailed mechanisms of arrhythmia and its treatment methods, it is required to elucidate the mechanisms of formation and debacle of cardiac beat at a microscopic level by using appropriate <i>in vitro</i> models of cardiac beating. In this study, we have developed a method for an medium-exchange-free culture of rat cardiac myocytes, and carried out long-term recording of electrical activity of cardiac myocytes from postnatal rat by means of microelectrode-array (MEA) and the new culture method. Figure 6 a) ∼ f) is a series of the properties of spontaneous beating (mean beating interval, standard deviation and coefficient of variation) of cardiac myocytes cultured by conventional culture method with alternate-day medium exchange (a ∼ c) or newly developed "molecular diffusion culture" method (d ∼ f). As shown in these figures, the difference of the beating property between the individual cultures, which is observed in conventional cultures (a) ∼ c)), was dramatically reduced in the molecular diffusion cultures (d) ∼ f)). In addition, these results also indicated that there is a transient period when all of mean beating interval, standard deviation, and coefficient of variation increase transiently before the formation of stable rhythmic fast beat.

収録刊行物

  • 電気学会論文誌. C, 電子・情報・システム部門誌 = The transactions of the Institute of Electrical Engineers of Japan. C, A publication of Electronics, Information and System Society  

    電気学会論文誌. C, 電子・情報・システム部門誌 = The transactions of the Institute of Electrical Engineers of Japan. C, A publication of Electronics, Information and System Society 128(7), 1064-1069, 2008-07-01 

    The Institute of Electrical Engineers of Japan

参考文献:  6件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  1件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021133030
  • NII書誌ID(NCID)
    AN10065950
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    03854221
  • NDL 記事登録ID
    9563981
  • NDL 雑誌分類
    ZN31(科学技術--電気工学・電気機械工業)
  • NDL 請求記号
    Z16-795
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ