Fabrication of DEP Device for Cell Positioning and its Cell Viability Test

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Studies pertaining cell chips require a cell positioning technique for the application of cell engineering such as single cell analysis, etc. Dielectrophoresis (DEP) is one of the promising methods to realize simultaneous multi-cell positioning. Previously, several research groups performed DEP-based cell manipulation, for example, cell separation, sorting, and positioning. In this study, we verify cell viability after positioning by DEP. We have fabricated a saw-shaped electrode on a glass chip and demonstrated simultaneous multi-cell positioning wherein cells are arranged between electrodes using the negative DEP principle. We describe the designs of two types of electrodes as well as their electric field simulations. The test chips were fabricated by the standard microelectromechanical system (MEMS) technology, and the experiments were performed for both polystyrene beads and living cells. To investigate the influence of DEP on the cell, the viability of the cells after the positioning was examined using a fluorescent dye. The results of this examination confirmed that no cytotoxicity was observed in the case of negative DEP positioning. Consequently, it was shown that the principle of negative DEP can be applied to achieve cell positioning without having a hazardous effect on living cells.

収録刊行物

  • 電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society  

    電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society 128(2), 59-63, 2008-02-01 

    The Institute of Electrical Engineers of Japan

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各種コード

  • NII論文ID(NAID)
    10021134975
  • NII書誌ID(NCID)
    AN1052634X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13418939
  • NDL 記事登録ID
    9361431
  • NDL 雑誌分類
    ZN31(科学技術--電気工学・電気機械工業)
  • NDL 請求記号
    Z16-B380
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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