A Combined Conjugation and Hybridization Technology for Different Types of DNA and Nanoparticles

この論文にアクセスする

この論文をさがす

著者

抄録

This study develops a new technology for conjugation between surface-modified Fe<SUB>3</SUB>O<SUB>4</SUB> nanoferrofluid and DNA that can be applied for biological sensors. After fabrication by chemical disposition and thorough washing, centrifuging and sieving, pure Fe<SUB>3</SUB>O<SUB>4</SUB> nanoferrofluid with mean particle size of 15 nm was obtained. PVP-k30 (Polyvinyl pyrrolidone) acts as dispersant to maintain a stable suspension of the Fe<SUB>3</SUB>O<SUB>4</SUB> nanoferrofluid, which is then added to and fully mixed with deionized water (ddwater) (pH >7). Experimental evidence shows that the surfaces of the Fe<SUB>3</SUB>O<SUB>4</SUB> particles carry positive charge and can effectively create a conjugating reaction with DNA. In addition, this TiO<SUB>2</SUB> nanofluid with mean particle size of 20 nm and high suspension stability is used to conjugate with another type of DNA. Finally, two sets of fluids, Fe<SUB>3</SUB>O<SUB>4</SUB> and TiO<SUB>2</SUB>, that have undergone conjugating reactions with different types of DNA are mixed in a culturecell, so they are linked together through the hybridization between different types of DNA. By spectrophotometer analysis, it is shown that DNA still exists in the fluid after hybridization, verifying that the hybridization technology between different types of DNA developed in this study is very successful.

収録刊行物

  • Materials transactions  

    Materials transactions 49(6), 1467-1473, 2008-06-01 

    The Japan Institute of Metals and Materials

参考文献:  19件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021147913
  • NII書誌ID(NCID)
    AA1151294X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13459678
  • NDL 記事登録ID
    9519609
  • NDL 雑誌分類
    ZP41(科学技術--金属工学・鉱山工学)
  • NDL 請求記号
    Z53-J286
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ