A Combined Conjugation and Hybridization Technology for Different Types of DNA and Nanoparticles
Access this Article
Search this Article
This study develops a new technology for conjugation between surface-modified Fe<SUB>3</SUB>O<SUB>4</SUB> nanoferrofluid and DNA that can be applied for biological sensors. After fabrication by chemical disposition and thorough washing, centrifuging and sieving, pure Fe<SUB>3</SUB>O<SUB>4</SUB> nanoferrofluid with mean particle size of 15 nm was obtained. PVP-k30 (Polyvinyl pyrrolidone) acts as dispersant to maintain a stable suspension of the Fe<SUB>3</SUB>O<SUB>4</SUB> nanoferrofluid, which is then added to and fully mixed with deionized water (ddwater) (pH >7). Experimental evidence shows that the surfaces of the Fe<SUB>3</SUB>O<SUB>4</SUB> particles carry positive charge and can effectively create a conjugating reaction with DNA. In addition, this TiO<SUB>2</SUB> nanofluid with mean particle size of 20 nm and high suspension stability is used to conjugate with another type of DNA. Finally, two sets of fluids, Fe<SUB>3</SUB>O<SUB>4</SUB> and TiO<SUB>2</SUB>, that have undergone conjugating reactions with different types of DNA are mixed in a culturecell, so they are linked together through the hybridization between different types of DNA. By spectrophotometer analysis, it is shown that DNA still exists in the fluid after hybridization, verifying that the hybridization technology between different types of DNA developed in this study is very successful.
- Materials Transactions, JIM
Materials Transactions, JIM 49(6), 1467-1473, 2008-06-01
The Japan Institute of Metals and Materials