古代青銅鏡の腐食層内に潜伏する微生物の検出とその同定 Detection and Identification of the Latent Microorganisms in the Corrosion Layers of Ancient Bronze Mirrors

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  On an ancient bronze mirror, we carried out a scanning electron microscopic (SEM) examination of fractured corrosion layers, a biological microscopic observation of a gram-stained sample and DNA analyses of the samples removed from corroded sites.<br>    From these scientific and technical researches, we obtained the following results.<br>    Fine particles about 2 μm in length were confirmed in the corrosion layer by the SEM observation. Microorganisms of the same size were observed in the gram-stained sample removed from the corrosion layer through a biological microscope. From these results, fine particles observed under the SEM should be considered as microorganisms. A lot of fine particles were confirmed especially in the corroded sites by the SEM observation. So (certain kinds of) microorganisms may have played a role in the corrosion (deterioration) of the ancient bronze mirrors while the mirrors were buried in soil.<br>    From the base sequences obtained by DGGE analysis, two kinds of microorganisms were confirmed to exist in the corrosion layer of the ancient bronze mirror. One is 97.4% homologous to the 16S rDNA of the uncultured bacterium (Accession number: AY 053488). It was also highly homologous to the sequence derived from the 16S rDNA of the <i>Xanthomonadaceae</i> family such as <i>Stenotrophomonas and Xanthomona.</i> That is, the sequence is derived from a strain belonging to the <i>Xanthomonadaceae</i> family. The other base sequence is 97.4% homologous to the 16S rDNA of the <i>Bacteroidales</i> order such as uncultured <i>Bacteroidales bacterium</i> (Accession number: AY 859647). That is, the sequence is derived from a strain belonging to the <i>Bacteroidales</i> order.<br>    Genes of microorganisms, presumed to belong to the <i>Acetobacter</i>, <i>Gluconacetobacter genuses</i> and Fe(III)-reducing bacterium, <i>Shewanella algae</i> were detected from the base sequence analysis by cloning.<br>    From the results above, microbial activity is assumed to be high around the ancient bronze mirrors. The corrosion mechanism of bronze mirrors seems complicated, but a several types of microbes which possibly corrode bronze mirrors were verified.<br>

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  • 日本金屬學會誌 = Journal of the Japan Institute of Metals  

    日本金屬學會誌 = Journal of the Japan Institute of Metals 72(2), 111-116, 2008-02-01 

    The Japan Institute of Metals

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各種コード

  • NII論文ID(NAID)
    10021149691
  • NII書誌ID(NCID)
    AN00062446
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00214876
  • NDL 記事登録ID
    9371304
  • NDL 雑誌分類
    ZP41(科学技術--金属工学・鉱山工学)
  • NDL 請求記号
    Z17-314
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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