高温接触酸化型トイレ実証試験における処理槽内の細菌群集構造解析 Analysis of Bacterial Community Structure in Composting Toilet Reactors Using Molecular Biological Techniques

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The bacterial community structure in composting toilet reactors was analyzed using three molecular biological techniques (PCR-DGGE, T-RFLP and cloning). Investigation was conducted for about six months at two trial sites in Japan. The main points of difference between the two sites are in the frequency of use air temperature (Site1: 5-6 times·d<sup>-1</sup>, 14.9°C, Site2: 30 times·d<sup>-1</sup>, 23.4°C). The DGGE band pattern indicated that the bacterial community profiles changed immediately after the start of the operation and did not reach a stable phase. Sequencing of the dominant DGGE band and clones revealed that the members of the community consisted of <i>Bacteroidetes</i>-related, <i>Proteobacteria</i>-related and other uncultured bacteria in the Site1 reactor. In contrast, <i>Bacillus</i>-related bacteria were detected from dominant DGGE bands from samples in Site2. This difference resulted from the difference in temperature inside the reactors. Some sequences from Site2 samples were also reported from other biodegradation processes under aerobic and thermophilic conditions. The bacterial community of the Site1 profile also had some similarity to other composting processes. Further investigation into the details of the bacterial community and the metabolic characteristics is needed to improve composting toilet operation.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment  

    水環境学会誌 = Journal of Japan Society on Water Environment 31(5), 253-259, 2008-05-10 

    Japan Society on Water Environment

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各種コード

  • NII論文ID(NAID)
    10021158599
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    9506666
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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