Operation of a Methanol Denitrification Reactor Fed with NO3- and NO2- as Electron Acceptors and Analysis of Denitrifying Bacterial Genes

• NITTAMI Tadashi

  Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University

• IMAI Yuko

  Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University

• NAKAMURA Kazuho

  Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University

• MATSUMOTO Kanji

  Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University

Bibliographic Information

Other Title

• ＮＯ３｀－´およびＮＯ２｀－´を電子受容体としたメタノール脱窒リアクターの運転とその脱窒細菌の遺伝子の解析
• NO3[-]およびNO2[-]を電子受容体としたメタノール脱窒リアクターの運転とその脱窒細菌の遺伝子の解析
• NO3 オヨビ NO2 オ デンシ ジュヨウタイ ト シタ メタノール ダッチツ リアクター ノ ウンテン ト ソノ ダッチツ サイキン ノ イデンシ ノ カイセキ
• Operation of a methanol denitrification reactor fed with NO3− and NO2− as electron acceptors and analysis of denitrifying bacterial genes

Abstract

We compared the denitrification rates and bacterial denitrification genes (nirK and nirS) between denitrification processes using NO₂^- and NO₃^-. We operated a sequencing batch reactor (SBR) for denitrification using activated sludge. The SBR was first fed with NO₃^- (Run 1), and the electron acceptor was then changed to NO₂^- (Run 2). Methanol was fed as the major electron donor through out the operational period (64 days). Denitrification rates (mg-N · g-MLSS^-1 · h^-1) were measured at regular intervals. Results showed that the maximum denitrification rates were more than 40 mg-N · g-MLSS^-1 · h^-1 irrespective of electron acceptor type. However, it takes 12 days to reach the maximum denitrification rate after changing the electron acceptor to NO₂^-. The results of the cloning analysis of nirK and nirS implied that the lag time was attributable to bacterial population shifts, because the nirK and nirS detected at Run 1 were phylogenetically different from those at Run 2. However, the changes in the total copy numbers of nirK or nirS could not explain the changes in the denitrifying rates.

Journal

• Journal of Japan Society on Water Environment

  Journal of Japan Society on Water Environment 31 (5), 269-274, 2008

  Japan Society on Water Environment

Keywords

• activated sludge
• cloning
• nitrite denitrification
• nitrite reductase gene (<I>nir</I>)
• real-time PCR

Details

• CRID: 1390001205082375168
• NII Article ID: 10021158660
• NII Book ID: AN10372439
• DOI: 10.2965/jswe.31.269
• COI: 1:CAS:528:DC%2BD1cXmvVOltb4%3D
• ISSN: 18813690
• ISSN: 09168958
• NDL BIB ID: 9506726
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9506726
• Web Site: https://www.jstage.jst.go.jp/article/jswe/31/5/31_5_269/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed