Electrostatic Force Imaging of Nano-scale Adsorbates on Insulating Substrates-Application to Biological Molecules-

• MATSUMOTO Takuya

  Institute of Scientific and Industrial Research, Osaka University

• YAMADA Fumihiko

  Institute of Scientific and Industrial Research, Osaka University

• MIKAMO-SATOH Eriko

  Institute of Scientific and Industrial Research, Osaka University Department of Pharmacy, Hyogo University of Health Sciences

• TAKAGI Akihiko

  Institute of Scientific and Industrial Research, Osaka University Institute of Physical and Chemical Research (RIKEN)

• KAWAI Tomoji

  Institute of Scientific and Industrial Research, Osaka University

Bibliographic Information

Other Title

• 「表面界面のナノ力学」絶縁体表面における吸着ナノ物質の静電気力イメージング―バイオ分子への応用―
• 絶縁体表面における吸着ナノ物質の静電気力イメージング--バイオ分子への応用
• ゼツエンタイ ヒョウメン ニ オケル キュウチャク ナノ ブッシツ ノ セイデンキリョク イメージング バイオ ブンシ エノ オウヨウ
• —Application to Biological Molecules—
• —バイオ分子への応用—

Abstract

We report here surface potential and differential electrostatic force images of DNAs, proteins, and gold nanoparticles on insulating substrates. The frequency-shift mode was essential for these experiments to satisfy enough sensitivity to use indirect modulation and to avoid unexpected charge injection into insulating substrate surface caused by tip-sample contact. It is obvious that the surface potential of insulating substrate is indefinable and influenced by treatment process and conditions. However, the potential difference between adsorbates and substrate surface is meaningful, reflecting charge or dipoles of adsorbates. We demonstrated that electrostatic force microscopy provides characteristic contrast inversion between DNA and transcription complex images reflecting the difference of electric polarizability of these molecules. These findings indicate that the electrostatic properties of individual biological molecules can be imaged on an insulator substrate during the retaining complex formation.

Journal

• Hyomen Kagaku

  Hyomen Kagaku 29 (4), 246-252, 2008

  The Surface Science Society of Japan

Keywords

• insulating substrate
• surface potential microscopy
• electrostatic force microscopy
• DNA
• transcription complex

Details

• CRID: 1390282681434568704
• NII Article ID: 130004486491
• NII Article ID: 10021165913
• NII Book ID: AN00334149
• DOI: 10.1380/jsssj.29.246
• ISSN: 18814743
• ISSN: 03885321
• NDL BIB ID: 9469517
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9469517
• Web Site: http://www.jstage.jst.go.jp/article/jsssj/29/4/29_4_246/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed