マイクロ流体デバイスによる脂質2重膜の形成 Formation of Bilayer Lipid Membranes with Microfluidic Devices

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Membrane proteins play very important roles in cells. They are also useful in various industrial fields, including drug discovery, and highly sensitive ion-channel-based biosensors. Here, I describe our two approaches toward membrane protein chips: an array of membrane proteins on (1) planar lipid bilayers and (2) giant vesicles. First, a highly reproducible method was developed for planar lipid bilayer reconstitution. Planar lipid bilayers are formed at apertures by flowing lipid organic solution and buffer alternately into an integrated microfluidic channel. Using this technique, multiple lipid bilayers are formed simultaneously, and channel currents through peptide ion channels were recorded. Second, I describe a method for the preparation of lipid vesicles inspired by forming soap bubbles from a soap film. In our "blowing" method, lipid vesicles are blown out of the pre-formed bio-functional planar lipid membrane, directly encapsulating ejected materials. We believe that these devices are useful for an efficient and rapid analysis of singlespecies-specific membrane proteins.

収録刊行物

  • 表面科学 = Journal of The Surface Science Society of Japan  

    表面科学 = Journal of The Surface Science Society of Japan 29(6), 370-374, 2008-06-10 

    The Surface Science Society of Japan

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各種コード

  • NII論文ID(NAID)
    10021166417
  • NII書誌ID(NCID)
    AN00334149
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    03885321
  • NDL 記事登録ID
    9545806
  • NDL 雑誌分類
    ZM35(科学技術--物理学)
  • NDL 請求記号
    Z15-379
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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