植物ポリケタイド合成酵素の触媒機能の制御 Engineering of Plant Polyketide Synthases

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著者

    • 阿部 郁朗 ABE Ikuro
    • 静岡県立大学薬学部, 科学技術振興機構PRESTO School of Pharmaceutical Sciences, University of Shizuoka, and PRESTO, Japan Science and Technology Agency

抄録

Recent progress of our research on the plant-specific type III polyketide synthases (PKSs) is described. First, our studies demonstrated that the enzymes accept a variety of substrate analogues to synthesize products chemically and structurally different from the natural products. Manipulation of the enzymes by artificial substrates could thus lead to development of a chemical library of pharmaceutically important novel polyketides. Second, a growing number of functionally divergent type III PKSs have been cloned and characterized, which include recently obtained pentaketide chromone synthase and octaketide synthase from the aloe plant. Structure function analysis revealed that a chemically inert single residue lining the active-site cavity determines the polyketide chain length and the product specificity. The functional diversity of type III PKSs is thus evolved from the simple steric modulation of the active-site cavity accompanied by conservation of a Cys-His-Asn catalytic triad. These results provided novel strategies for the engineered biosynthesis of plant polyketides.

収録刊行物

  • 有機合成化学協会誌 : JOURNAL OF Synthetic Organic Chemistry JAPAN  

    有機合成化学協会誌 : JOURNAL OF Synthetic Organic Chemistry JAPAN 66(7), 683-693, 2008-07-01 

    The Society of Synthetic Organic Chemistry, Japan

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各種コード

  • NII論文ID(NAID)
    10021176048
  • NII書誌ID(NCID)
    AN0024521X
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    00379980
  • NDL 記事登録ID
    9577743
  • NDL 雑誌分類
    ZP11(科学技術--化学・化学工業--有機化学・有機化学工業)
  • NDL 請求記号
    Z17-256
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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