Differentiation of pathogenic and nonpathogenic isolates of <i>Geotrichum candidum</i> sensu Suprapta et al. (1995) on citrus fruit based on PCR-RFLP analysis of rDNA ITS and PCR using specific primers designed in polygalacturonase genes
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- Nakamura Masayuki
- Faculty of Agriculture, Kagoshima University
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- Suprapta Dewa Ngurah
- Department of Plant Protection, Faculty of Agriculture, Udayana University
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- Iwai Hisashi
- Faculty of Agriculture, Kagoshima University
Bibliographic Information
- Other Title
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- Differentiation of pathogenic and nonpathogenic isolates of Geotrichum candidum sensu Suprapta et al. (1995) on citrus fruit based on PCR-RFLP analysis of rDNA ITS and PCR using specific primers designed in polygalacturonase genes
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Abstract
<p>Pathogenic and nonpathogenic isolates of Geotrichum candidum sensu Suprapta et al. (1995) that affect citrus fruit are indistinguishable morphologically. In this work, differentiation of the two pathogenicity types based on the internal transcribed spacer regions of ribosomal DNA (rDNA-ITS) and polygalacturonase (PG) genes was attempted. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of rDNA ITS and PCR using specific primers to PG genes from each type could clearly differentiate the two pathogenicity types, and the profiles by PCR-RFLP of rDNA ITS genes corresponded with those by type-specific PCR of PG genes. These results indicate that the two pathogenic types can be differentiated at a molecular level and that PG genes are alternatively useful for distinguishing between the two types.</p>
Journal
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- Mycoscience
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Mycoscience 49 (2), 155-158, 2008
The Mycological Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390295603315518208
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- NII Article ID
- 10021182570
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- NII Book ID
- AA11003436
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- ISSN
- 16182545
- 13403540
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- NDL BIB ID
- 9450961
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Allowed