Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells

この論文にアクセスする

この論文をさがす

著者

    • HIDA Tatsuhiro
    • Department of Conservative Dentistry, Osaka University Faculty of Dentistry
    • IKEDA Hiroko
    • Department of Anatomy and Histology, Fukushima Medical University, School of Medicine
    • KAMETAKA Satoshi
    • Department of Anatomy and Histology, Fukushima Medical University, School of Medicine
    • EBISU Shigeyuki
    • Department of Conservative Dentistry, Osaka University Faculty of Dentistry
    • UCHIYAMA Yasuo
    • Department of Cell Biology and Neuroscience (A1), Osaka University Graduate School of Medicine
    • WAGURI Satoshi
    • Department of Anatomy and Histology, Fukushima Medical University, School of Medicine

抄録

Three mammalian GGAs (Golgi-localized, γ-ear-containing, ARF-binding proteins), GGA1, 2, and 3 have been implicated in the sorting of mannose 6-phosphate receptor (MPR). To investigate the distinct roles of GGA2 in lysosomal enzyme transport, we established two stable cell lines that had a reduced expression of GGA2 by RNA interference. The expression levels of GGA2 were approximately 5% of the control levels, whereas those of non-targeted GGA1 and GGA3 were not apparently reduced. The depletion of GGA2 did not cause changes in the overall distribution of GGA1, GGA3, cation-dependent MPR, or cation-independent MPR. However, the cell lines showed increased secretion of a lysosomal enzyme, cathepsin D. In addition, a moderate expression of the dominant negative VHS-GAT domain of GGA2 had no effect on the trans-Golgi network (TGN) signal of three GGAs, nor was the GGA2 signal affected by the expression of VHS-GAT domain of GGA1 or 3. These results suggest that GGA2 is recruited to the TGN independently of the other GGAs and is required for the efficient sorting of lysosomal enzymes.

収録刊行物

  • Archives of histology and cytology  

    Archives of histology and cytology 70(5), 303-312, 2007-12-01 

    International Society of Histology and Cytology

参考文献:  28件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021186983
  • NII書誌ID(NCID)
    AA1068990X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09149465
  • データ提供元
    CJP書誌  J-STAGE 
ページトップへ