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- Hida Tatsuhiro
- Department of Conservative Dentistry, Osaka University Faculty of Dentistry
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- Ikeda Hiroko
- Department of Anatomy and Histology, Fukushima Medical University, School of Medicine
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- Kametaka Satoshi
- Department of Anatomy and Histology, Fukushima Medical University, School of Medicine
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- Akazawa Chihiro
- Department of Neurochemistry, National Institute of Neuroscience
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- Kohsaka Shinichi
- Department of Neurochemistry, National Institute of Neuroscience
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- Ebisu Shigeyuki
- Department of Conservative Dentistry, Osaka University Faculty of Dentistry
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- Uchiyama Yasuo
- Department of Cell Biology and Neuroscience (A1), Osaka University Graduate School of Medicine
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- Waguri Satoshi
- Department of Anatomy and Histology, Fukushima Medical University, School of Medicine
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抄録
Three mammalian GGAs (Golgi-localized, γ-ear-containing, ARF-binding proteins), GGA1, 2, and 3 have been implicated in the sorting of mannose 6-phosphate receptor (MPR). To investigate the distinct roles of GGA2 in lysosomal enzyme transport, we established two stable cell lines that had a reduced expression of GGA2 by RNA interference. The expression levels of GGA2 were approximately 5% of the control levels, whereas those of non-targeted GGA1 and GGA3 were not apparently reduced. The depletion of GGA2 did not cause changes in the overall distribution of GGA1, GGA3, cation-dependent MPR, or cation-independent MPR. However, the cell lines showed increased secretion of a lysosomal enzyme, cathepsin D. In addition, a moderate expression of the dominant negative VHS-GAT domain of GGA2 had no effect on the trans-Golgi network (TGN) signal of three GGAs, nor was the GGA2 signal affected by the expression of VHS-GAT domain of GGA1 or 3. These results suggest that GGA2 is recruited to the TGN independently of the other GGAs and is required for the efficient sorting of lysosomal enzymes.
収録刊行物
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- Archives of Histology and Cytology
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Archives of Histology and Cytology 70 (5), 303-312, 2007
国際組織細胞学会
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詳細情報
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- CRID
- 1390282681388724736
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- NII論文ID
- 10021186983
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- NII書誌ID
- AA1068990X
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- ISSN
- 13491717
- 09149465
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可