10-color FACS-based method of chimerism analysis after multi-cord blood transplantation

• Ishii Yumiko

  FACS Core Laboratory, The Institute of Medical Science, The University of Tokyo

• Takahashi Satoshi

  Reserch Hospital

• Watanabe Nobukazu

  FACS Core Laboratory, The Institute of Medical Science, The University of Tokyo

• Ishige Masayuki

  FACS Core Laboratory, The Institute of Medical Science, The University of Tokyo

• Nakauchi Hiromitsu

  Division of Stem Cell Therapy

Bibliographic Information

Other Title

• 複数臍帯血移植後の複合キメリズム解析を目的とした10カラーFACS解析システムの開発

Abstract

Cord blood transplantation (CBT) has become a standard therapeutic option in recent years. Compared with bone marrow transplantation (BMT), CBT has some advantages; rapid availability and low risk of severe acute graft-versus host disease (GVHD). However, CBT in adults is limited by graft cell dose. Multiple cord blood transplant (MCBT) in which two or more umbilical cord blood units are given is reported to enhance the engraftment compared with single unit CBT. However, little is known about the mechanism of enhanced engraftment. To elucidate the early engraftment kinetics of two units CBT, we developed the flow cytometry -based method of chimerism analysis using anti-HLA monoclonals.

Journal

• Cytometry Research

  Cytometry Research 18 (1), 31-34, 2008

  Japan Cytometry Society

Keywords

• multiple cord blood transplantation
• HLA-mismatch
• chimerism
• flow cytometry

Details

• CRID: 1390282679449602560
• NII Article ID: 130006832465
• NII Article ID: 10021194563
• NII Book ID: AN10356261
• DOI: 10.18947/cytometryresearch.18.1_31
• ISSN: 24240664
• ISSN: 09166920
• Web Site: https://search.jamas.or.jp/link/ui/2008180995
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed