体内および体外で成熟したマウス卵母細胞の囲卵腔の大きさと媒精後の多精子受精の頻度 Size of Perivitelline Space and Incidence of Polyspermy in Mouse Oocytes Matured In Vivo and In Vitro

この論文にアクセスする

この論文をさがす

著者

抄録

In mouse oocytes soon after collection, perivitelline space could be seen in about half of them and its mean size was 0.28 μm. After 6 hrs of in vivo and in vitro maturation, perivitelline space appeared in all oocytes. In oocytes in the process of maturation in vivo, the mean size of perivitelline space significantly increased 6 hrs after hCG injection and reached 7.00 μm at 14 hrs after the hCG injection. On the other hand, perivitelline space in cultured oocytes enlarged significantly at 2 hrs culture. Thereafter, perivitelline space in oocytes continued to enlarge with culture time and reached 5.40 μm at 14 hrs of maturation culture. The size of perivitelline space in oocytes 14 hrs after the hCG injection was significantly larger than that in oocytes cultured for 14 hrs. The fertilization rates of oocytes matured in vivo (85%) did not differ from that of oocytes matured in vitro (80%). However, the incidence of polyspermy in oocytes matured in vivo was 4%, which was significantly lower than the 16% of oocytes matured in vitro. These findings suggest there could be a relationship between the size of the perivitelline space and the incidence of polyspermy in mouse oocytes.

In mouse oocytes soon after collection, perivitelline space could be seen in about half of them and its mean size was 0.28 μm. After 6 hrs of in vivo and in vitro maturation, perivitelline space appeared in all oocytes. In oocytes in the process of maturation in vivo, the mean size of perivitelline space significantly increased 6 hrs after hCG injection and reached 7.00 μm at 14 hrs after the hCG injection. On the other hand, perivitelline space in cultured oocytes enlarged significantly at 2 hrs culture. Thereafter, perivitelline space in oocytes continued to enlarge with culture time and reached 5.40 μm at 14 hrs of maturation culture. The size of perivitelline space in oocytes 14 hrs after the hCG injection was significantly larger than that in oocytes cultured for 14 hrs. The fertilization rates of oocytes matured in vivo (85%) did not differ from that of oocytes matured in vitro (80%). However, the incidence of polyspermy in oocytes matured in vivo was 4%, which was significantly lower than the 16% of oocytes matured in vitro. These findings suggest there could be a relationship between the size of the perivitelline space and the incidence of polyspermy in mouse oocytes.<br>

収録刊行物

  • Journal of mammalian ova research = 日本哺乳動物卵子学会誌  

    Journal of mammalian ova research = 日本哺乳動物卵子学会誌 25(1), 44-49, 2008-04-01 

    日本哺乳動物卵子学会

参考文献:  20件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021217707
  • NII書誌ID(NCID)
    AN10548943
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417738
  • NDL 記事登録ID
    9523857
  • NDL 雑誌分類
    ZR4(科学技術--生物学--動物)
  • NDL 請求記号
    Z18-1673
  • データ提供元
    CJP書誌  NDL  IR  J-STAGE 
ページトップへ