ミニブタ精子抽出物を注入したブタ卵子におけるp34cdc2kinaseとMAP kinase活性および人為的活性化

DOI 機関リポジトリ 日本農学文献記事索引 Web Site 被引用文献2件 参考文献30件 オープンアクセス

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  • P34cdc2 Kinase and MAP Kinase Activities and Parthenogenetic Activation in Porcine Oocytes after Injection of Miniature Pig Sperm Extracts
  • P34<sup>cdc2</sup>Kinase and MAP Kinase Activities and Parthenogenetic Activation in Porcine Oocytes after Injection of Miniature Pig Sperm Extracts

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The aim of the present study was to examine the rate of activation and time-dependent changes in p34cdc2 kinase and MAP kinase activities in porcine oocytes after injection of sperm extracts (SE) or treatment with Ca2+ ionophore to clarify whether SE injection is useful for porcine oocyte activation. SE was prepared from miniature pig sperm by non-ionic surfactant. Oocytes that were treated with Ca2+ ionophore and injected with SE were activated at rates of 41% and 46%, respectively. The activities of p34cdc2 kinase and MAP kinase in each culture period (4, 8 and 12 h) in the Ca2+ ionophore group and SE injection group were significantly lower than those in the control group. These results suggest that SE injection induces decrease of p34cdc2 kinase and MAP kinase activity, and induces oocyte activation, as well as Ca2+ ionophore treatment, but the induction was comparatively limited.<br>

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