ミニブタ精子抽出物を注入したブタ卵子における p34^<cdc2> kinase と MAP kinase 活性および人為的活性化 P34^<cdc2> Kinase and MAP Kinase Activities and Parthenogenetic Activation in Porcine Oocytes after Injection of Miniature Pig Sperm Extracts

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The aim of the present study was to examine the rate of activation and time-dependent changes in p34cdc2 kinase and MAP kinase activities in porcine oocytes after injection of sperm extracts (SE) or treatment with Ca2+ ionophore to clarify whether SE injection is useful for porcine oocyte activation. SE was prepared from miniature pig sperm by non-ionic surfactant. Oocytes that were treated with Ca2+ ionophore and injected with SE were activated at rates of 41% and 46%, respectively. The activities of p34cdc2 kinase and MAP kinase in each culture period (4, 8 and 12 h) in the Ca2+ ionophore group and SE injection group were significantly lower than those in the control group. These results suggest that SE injection induces decrease of p34cdc2 kinase and MAP kinase activity, and induces oocyte activation, as well as Ca2+ ionophore treatment, but the induction was comparatively limited.

The aim of the present study was to examine the rate of activation and time-dependent changes in p34<sup>cdc2</sup> kinase and MAP kinase activities in porcine oocytes after injection of sperm extracts (SE) or treatment with Ca<sup>2+</sup> ionophore to clarify whether SE injection is useful for porcine oocyte activation. SE was prepared from miniature pig sperm by non-ionic surfactant. Oocytes that were treated with Ca<sup>2+</sup> ionophore and injected with SE were activated at rates of 41% and 46%, respectively. The activities of p34<sup>cdc2</sup> kinase and MAP kinase in each culture period (4, 8 and 12 h) in the Ca<sup>2+</sup> ionophore group and SE injection group were significantly lower than those in the control group. These results suggest that SE injection induces decrease of p34<sup>cdc2</sup> kinase and MAP kinase activity, and induces oocyte activation, as well as Ca<sup>2+</sup> ionophore treatment, but the induction was comparatively limited.<br>

収録刊行物

  • Journal of mammalian ova research = 日本哺乳動物卵子学会誌  

    Journal of mammalian ova research = 日本哺乳動物卵子学会誌 25(1), 63-68, 2008-04-01 

    日本哺乳動物卵子学会

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各種コード

  • NII論文ID(NAID)
    10021217798
  • NII書誌ID(NCID)
    AN10548943
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417738
  • NDL 記事登録ID
    9523956
  • NDL 雑誌分類
    ZR4(科学技術--生物学--動物)
  • NDL 請求記号
    Z18-1673
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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