The blastocyst production rate and incidence of chromosomal abnormalities by developmental stage in vitro produced porcine embryos

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  • ULLOA ULLO Carlos Manuel
    United Graduate School of Agriculture, Tokyo University of Agriculture and Technology Faculty of Agriculture, Utsunomiya University
  • YOSHIZAWA Midori
    United Graduate School of Agriculture, Tokyo University of Agriculture and Technology Faculty of Agriculture, Utsunomiya University
  • KOMORIYA Eiji
    Faculty of Agriculture, Utsunomiya University
  • MITSUI Akinori
    United Graduate School of Agriculture, Tokyo University of Agriculture and Technology Faculty of Agriculture, Utsunomiya University
  • NAGAI Takashi
    National Institute of Livestock and Grassland Science
  • KIKUCHI Kazuhiro
    Genetic Diversity Department, National Institute of Agrobiological Sciences

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タイトル別名
  • The Blastocyst Production Rate and Incidence of Chromosomal Abnormalities by Developmental Stage in In Vitro Produced Porcine Embryos

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The present study was conducted to determine the relationship between embryonic development speed at different stages (the cleaved stage at 52 h and the blastocyst stage at 6 days post insemination) and incidences of chromosome abnormalities in in vitro produced porcine embryos. Porcine oocytes were collected from 3-6-mm ovarian follicles obtained at a slaughterhouse and matured in modified NCSU-37 medium for 44-46 h. Following in vitro fertilization with a final concentration of 1 × 105 sperm/ml for 3 h, all oocytes were cultured in vitro for 52 h. Day-2 (52 h after insemination) embryos were classified according to their cleaved stages into 2-cell, 3- to 4-cell, 5- to 8-cell, and >8-cell stages; these were cultured separately for additional 4 days (Day 6). The resultant Day-6 blastocysts were classified according to the morphological diameter into 3 grades: Grade A, expanded blastocysts; Grade B, expanding blastocysts; and Grade C, early blastocysts. They were then analyzed chromosomally. The 3- to 4-cell and 5- to 8-cell embryos had significantly high blastocyst development rates (46.1 and 36.9%, respectively), and these blastocysts contained significantly more cells (40.2 and 42.4 cells, respectively) than those derived from 2-cell embryos and >8-cell embryos (28.6 and 26.5 cells, respectively). The incidence of chromosomal abnormalities was significantly higher in the blastocysts derived from 2-cell and >8-cell stage embryos than in the blastocysts derived from the other stage embryos. Furthermore, the grade A blastocysts had the lowest incidence of chromosomal abnormalities (35.3%) and contained the most cells (48.7 cells). Porcine in vitro production (IVP) yielded a high blastocyst rate and an excellent embryo quality when 3- to 4-cell and 5- to 8-cell stage embryos were selected on Day 2 after insemination. The same criteria yielded a higher quality of expanded blastocysts based on the stage of embryo development and morphology.<br>

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