Effect of Volume of Non-Surgical Embryo Transfer Medium on Ability of Porcine Embryos to Survive to Term

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Non-surgical embryo transfer is a promising method for improving efficiency in the pork industry and also for biotechnology applications, such as <i>in vitro</i> embryo production, transgenesis and cloning. Several groups have reported successful piglet production using an artificial insemination catheter or flexible catheter designed for this procedure; however, the efficiency of the technique is still low. The critical points that need to be addressed in order to improve this procedure are (1) the embryo deposition site and (2) volume of transfer medium associated with the embryos; however, the latter has not yet been examined systematically. In the present study, we evaluated the effect of the volume of non-surgical embryo transfer medium on the ability of porcine embryos to survive to term by using a recently produced flexible catheter. The catheter consists of a guide and an injector. Blastocysts 200-230 <i>μ</i>m in diameter were collected from donor gilts and transferred to recipient gilts. The time required for the completion of embryo transfer using this catheter was 14.6 ± 3.9 min. The tip of the injector was determined by laparotomy to be located in a uterine horn 20-30 cm anterior from the branching point of the uterus body. We transferred 17.0-17.3 embryos with different volumes of medium (1.6, 3.2 and 10 ml) into each of 5, 4 and 4 recipients, respectively, and pregnancy was confirmed in 4, 3 and 1 of these recipients, respectively. Three recipients in the 1.6 ml group farrowed a total of 19 piglets (4, 5 and 10 piglets, respectively). These results suggest that successful non-surgical embryo transfer is affected by the volume of transfer medium.<br>

収録刊行物

  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(1), 30-34, 2008-02-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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各種コード

  • NII論文ID(NAID)
    10021218409
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168818
  • NDL 記事登録ID
    9395169
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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