Determination of the Estrogenic Activity of Wild Phytoestrogen-rich Pueraria mirifica by MCF-7 Proliferation Assay

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The aim of this study was to evaluate the estrogenic activity of tuberous samples of wild, phytoestrogen-rich <i>Pueraria mirifica</i> collected from 28 out of 76 provinces of Thailand by MCF-7 proliferation assay. The plant extracts were administered to MCF-7, ERα positive human mammary adenocarcinoma cell cultures, for 3 days at dosages of 0.1, 1, 10, 100 and 1,000 <i>μ</i>g/ml and were compared with 17<i>β</i>-estradiol at concentrations of 10<sup>-12</sup>-10<sup>-6</sup> M. The mean <i>P. mirifica</i> population at 1 <i>μ</i>g/ml exhibited significant proliferation. Two plant samples exhibited levels of proliferation in MCF-7 that were similar to 17<i>β</i>-estradiol. The mean <i>P. mirifica</i> populations at 100 and 1,000 <i>μ</i>g/ml exhibited significant cytotoxicity in MCF-7. Analysis of the estrogenic activity of puerarin, representative of major isoflavonoids in <i>P. mirifica</i> tubers, revealed proliferation in MCF-7 only at the highest dose (10<sup>-6</sup> M) that was 10<sup>2</sup>-10<sup>5</sup> times less active than 17<i>β</i>-estradiol. Puerarin and 17<i>β</i>-estradiol at concentration of 10<sup>-12</sup>-10<sup>-6</sup> M exhibited no cytotoxicity in MCF-7.<br>

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  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(1), 63-67, 2008-02-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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各種コード

  • NII論文ID(NAID)
    10021218595
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168818
  • NDL 記事登録ID
    9395349
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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