Cytochrome c Upregulation during Capacitation and Spontaneous Acrosome Reaction Determines the Fate of Pig Sperm Cells : Linking Proteome Analysis

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著者

    • SONG Hyuk
    • Department of Animal Science, College of Natural Science, Konkuk University
    • PARK Jin-Ki
    • Animal Biotechnology Division, National Livestock Research Institute, RDA
    • KIM Teoan
    • Department of Physiology, Catholic University of Daegu School of Medicine
    • PARK Chankyu
    • Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University
    • KIM Jin-Hoi
    • Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University

抄録

To identify the mechanisms underlying capacitation, we undertook a high-resolution differential proteomic analysis of pig sperm cells. Two-dimensional gel electrophoresis and subsequent MALDI-TOF mass spectrometry analyses led to identification of 56 differentially expressed proteins. After induction of capacitation <i>in vitro</i>, the well-established markers of the capacitation (lactadherin P47, acrosomal protein SP-10 precursor, prohibitin, proteasomes, DJ-1 protein and arylsulfatase-A) and TCA cycle proteins (isocitrate dehydrogenase, malate dehydrogenase and pyruvate dehydrogenase) were identified. During induction, cytochrome c expression via the p53 pathway increased, however apoptotic executors, such as caspase-3, decreased significantly. Therefore, we tested the hypothesis that cytochrome c upregulation in spermatozoa is capable of activating tyrosine phosphorylation for capacitation, rather than apoptosis. Exposure of sperm cells to soluble Na<sub>2</sub>CrO<sub>4</sub> [Cr (VI)], which induces cytochrome c upregulation, caused a dose- and time-dependent increase in tyrosine phosphorylation of sperm proteins in non-capacitating medium. In contrast, supplementation of cyclosporin A, which blocks cytochrome c upregulation, inhibited tyrosine phosphorylation of sperm proteins. Furthermore, spermatozoa in capacitation medium or non-capacitation media supplemented with soluble Cr (VI) showed similar levels of capacitation. These findings indicate that differential expression of many of these proteins has previously been unrecognized in sperm cells incubated in capacitation medium also suggest that a gradual increase of cytochrome c during incubation to induce capacitation determines sperm cell fate, i.e., apoptosis or further development for fertilization.<br>

収録刊行物

  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(1), 68-83, 2008-02-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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各種コード

  • NII論文ID(NAID)
    10021218630
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168818
  • NDL 記事登録ID
    9395372
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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