Piezo-assisted In Vitro Fertilization of Mouse Oocytes with Spermatozoa Retrieved from Epididymides Stored at 4 Degree C

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著者

    • Fan Zhi-Qiang FAN Zhi-Qiang
    • Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
    • Li Xiu-Wei LI Xiu-Wei
    • Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
    • Liu Ying [他] LIU Ying
    • Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
    • MENG Qing-Gang
    • Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
    • WANG Yan-Ping
    • Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
    • HOU Yun-Peng
    • National Key Laboratories for Agricultural Biotechnology, China Agricultural University
    • ZHOU Guang-Bin
    • Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
    • ZHU Shi-En
    • Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University

抄録

This study was performed to investigate the effect of partial zona pellucida incision by piezo micromanipulation (ZIP) on the <i>in vitro</i> fertilizing ability of stored mouse spermatozoa. The storage conditions were optimized by storing the mouse epididymides at 4 C in mineral oil or in the mouse body for up to 4 days after death, and the retrieved spermatozoa were used to fertilize fresh oocytes. No significant difference was observed in fertilization rates between the treatments when epididymides were stored for up to 2 days, but the fertilization rates in mineral oil were higher (P<0.05) than those in the mouse body at 3 (41.4 <i>vs.</i> 16.2%) and 4 days (26.0 <i>vs.</i> 15.8%). Spermatozoa retrieved from epididymides stored in mineral oil were then used to fertilize fresh and vitrified oocytes with or without ZIP treatment. The fertilization rates of the ZIP fresh oocytes were higher than those of the zona-intact oocytes at each time point (1 to 4 days). After ZIP, the fertilization rates of spermatozoa stored for 1 and 2 days (91.2 and 86.6%, respectively) were similar (P>0.05) to that of fresh spermatozoa (91.9%). In regard to vitrified oocytes, the fertilization rates of zona-intact and ZIP oocytes using fresh spermatozoa were 46.7 and 84.7%, while the fertilization rates of vitrified ZIP oocytes using spermatozoa stored for 1 to 4 days ranged from 49.3 to 79.6%. When 2-cell embryos derived from ZIP fresh and vitrified oocytes inseminated with 2 day-stored spermatozoa were transferred into recipient females, 47.9 and 15.0% of the embryos developed to term, respectively. These results indicate that storing mouse epididymides at 4 C in mineral oil is more suitable than storage in the mouse body and that the ZIP technique improves the <i>in vitro</i> fertilizing ability of stored mouse spermatozoa in fresh oocytes and significantly increases the fertilization rate of vitrified oocytes with fresh spermatozoa.<br>

収録刊行物

  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(2), 107-112, 2008-04-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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各種コード

  • NII論文ID(NAID)
    10021218827
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168818
  • NDL 記事登録ID
    9470104
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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