Interspecies Nuclear Transfer Embryos Reconstructed from Cat Somatic Cells and Bovine Ooplasm

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著者

    • INABA Yasushi
    • Embryo Transfer and In Vitro Fertilization Section, National Livestock Breeding Center
    • TASAI Mariko
    • Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Science
    • TAGAMI Takahiro
    • Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Science
    • NIRASAWA Keijiro
    • Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Science
    • NAGAI Takashi
    • Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Science
    • SAITO Norio
    • Embryo Transfer and In Vitro Fertilization Section, National Livestock Breeding Center
    • TECHAKUMPHU Mongkol
    • Department of Obstetrics, Gynecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University
    • TAKEDA Kumiko
    • Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Science

抄録

This study was conducted to investigate the developmental capacity of domestic cat-bovine reconstructed embryos via interspecies somatic cell nuclear transfer (iSCNT) and to observe the mitochondrial DNA (mtDNA) content of the iSCNT embryos. The iSCNT embryos were generated using mixed-breed domestic cat fibroblasts as donor cells and enucleated bovine oocytes as the recipient cytoplasm. When the developmental capacities of iSCNT embryos and parthenogenic bovine embryos were compared, there was no difference (P>0.05) in the rates of cleavage and development to the 8-cell stage (86.6 <i>vs.</i> 84.0% and 32.2 <i>vs.</i> 36.2%, respectively). However, in contrast to development of parthenogenic embryos to the morula and blastocyst stages, no iSCNT embryos (0/202) developed beyond the 8-cell stage. For mtDNA analysis, iSCNT embryos at the 1-cell, 2-cell, 4-cell and 8-cell stages were randomly selected. Both cat and bovine mtDNA quantification analysis were performed using quantitative PCR. The levels of both cat and bovine mtDNA in cat-bovine iSCNT embryos varied at each stage of development. The cat mtDNA concentration in the iSCNT embryos was stable from the 1-cell to 8-cell stages. The bovine mtDNA in the iSCNT embryos at the 8-cell stage was significantly lower than that at the 4-cell stage (P<0.05). No difference in the proportions of cat mtDNA in the iSCNT embryos was found in any of the observed developmental stages (1- through 8-cell stages). In conclusion, bovine cytoplasm supports domestic cat nucleus development through the 8-cell stage. The mtDNA genotype of domestic cat-bovine iSCNT embryos illustrates persistence of heteroplasmy, and the reduction in mtDNA content might reflect a developmental block at the 8-cell stage.<br>

収録刊行物

  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(2), 142-147, 2008-04-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

参考文献:  33件

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各種コード

  • NII論文ID(NAID)
    10021219040
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168818
  • NDL 記事登録ID
    9470180
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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