Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro

DOI Web Site Web Site 2 Citations 85 References
  • SOMFAI Tamás
    Division of Animal Sciences, National Institute of Agrobiological Sciences National Livestock Breeding Center
  • KASHIWAZAKI Naomi
    Graduate School, Azabu University
  • OZAWA Manabu
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • NAKAI Michiko
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • MAEDOMARI Naoki
    Graduate School, Azabu University
  • NOGUCHI Junko
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • KANEKO Hiroyuki
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • NAGAI Takashi
    National Institute of Livestock and Grassland Science
  • KIKUCHI Kazuhiro
    Division of Animal Sciences, National Institute of Agrobiological Sciences

Search this article

Abstract

The aim of the present study was to investigate the effects of centrifugation pretreatment on the viability and nuclear status of porcine in vitro matured (IVM) oocytes and on the developmental competence of in vitro fertilized (IVF) oocytes (zygotes) after cryopreservation by vitrification (Solid Surface Vitrification; SSV). Mature oocytes having the first polar body after IVM and zygotes having the second polar body at 10 h after IVF were centrifuged at 10,000 × g at 37 C for 20 min and then subjected to SSV. Their viability was evaluated by morphological appearance and fluorescein diacetate staining. The nuclear status of oocytes was evaluated 6 h after vitrification. The developmental ability to the blastocyst stage of vitrified zygotes was evaluated after 6 days of in vitro culture. Although centrifugation did not damage the oocytes directly, it drastically reduced the rate of live oocytes after SSV. The rates of vitrification-induced parthenogenetic activation were similar in both centrifuged and non-centrifuged oocytes (42.4 and 47.4%, respectively). Centrifugation had no significant effects on the viability of pronuclear oocytes. The development of vitrified zygotes to the blastocyst stage was significantly lower than that of the control irrespective of centrifugation pretreatment. There was no difference in the cleavage and blastocyst rates between the control and centrifuged zygotes after vitrification. There was also no difference in the total cell numbers of blastocysts between the control and centrifuged zygotes irrespective of vitrification. These results reveal that, in IVM porcine oocytes, centrifugation pretreatment is highly detrimental to cryotolerance; however, in zygotes, it has only a slight effect on viability and does not alter the developmental competence of surviving zygotes.<br>

Journal

Citations (2)*help

See more

References(85)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top