Effects of Ginsenosides on Organogenesis and Expression of Glutathione Peroxidase Genes in Cultured Rat Embryos

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著者

    • Lee Se-Ra LEE Se-Ra
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
    • Kim Mi-Ra KIM Mi-Ra
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
    • BAEK In-Jeoung
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
    • LEE Beom-Jun
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
    • AHN Byeongwoo
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
    • KIM Yun-Bae
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
    • YUN Young-Won
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
    • NAM Sang-Yoon
    • College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University

抄録

Ginseng has been extensively used around the world for several thousand years as a food or drug. However, recently, several reports have indicated that the organogenesis of cultured embryos is inhibited by treatment with ginsenoside, the principal component of ginseng. In this study, we evaluated the morphological changes of embryos and the gene expression patterns of antioxidant enzymes, 3 types of glutathione peroxidases [<i>GPx</i>; cytosolic (<i>cGPx</i>), plasma (<i>pGPx</i>) and phospholipid hydroperoxide (<i>phGPx</i>) forms], in cultured rat embryos (embryonic days 9.5-11.5) exposed to ginsenosides Rb1, Rg1, Re and Rc at levels of 5, 50 and 100 μg/ml. With regard to total morphological scores, no significant differences were noted in the embryos exposed to all doses of ginsenosides, with the exception of 50 μg/ml of Rc. In the cultured embryos exposed to Rg1, a majority of the developmental parameters were normal, but growth of the hind- and mid- brains and the caudal neural tube was significantly increased compared with that observed in the control group (P<0.05). Furthermore, Rc significantly enhanced the growth of a variety of developmental parameters in the cultured embryos, with the exception of the hindlimbs. According to the results of our semiquantitative RT-PCR analysis, the levels of <i>cGPx</i> and <i>phGPx</i> mRNA in the cultured embryos were unaffected by treatment with the ginsenosides. However, the levels of <i>pGPx</i> mRNA increased significantly in the embryos treated with ginsenosides Re, Rc and Rb1 compared with the control group (P<0.05). These findings indicate that ginsenosides may exert a stimulatory effect on the growth of embryos via differential expression of <i>GPx</i> genes.<br>

収録刊行物

  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(3), 164-170, 2008-06-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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各種コード

  • NII論文ID(NAID)
    10021219175
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168818
  • NDL 記事登録ID
    9542974
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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