Identification of ZAG1, a Novel Protein Expressed in Mouse Preimplantation, and Its Putative Roles in Zygotic Genome Activation

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著者

    • Sato Manabu SATO Manabu
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • SHIN Seung-Wook
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • UENOYAMA Atsuto
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • ITO Kazunari
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • HITOMI Syuji
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • AMANO Tomoko
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • SAEKI Kazuhiro
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • HOSOI Yoshihiko
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • IRITANI Akira
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University
    • MATSUMOTO Kazuya
    • Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University

抄録

We isolated a mouse cDNA, <i>zag1</i> (zygotic gene activation-associated gene 1), that has an open reading frame of 1,728-bp encoding a protein of 66.2 kDa including both a bipartite nuclear targeting sequence and a P-loop motif containing nucleoside triphosphate hydrolase motifs. Northern blot analysis of mouse tissues showed that <i>zag1</i> was widely expressed but was especially prominent in the ovary and testis. RT-PCR analysis of <i>in vitro</i> fertilized embryos showed that the abundance of <i>zag1</i> transcripts in oocytes decreased after fertilization, and <i>zag1</i> mRNA was detected at 15 h post insemination (hpi) in fertilized embryos indicating that the gene was expressed at the start of zygotic gene activation at the mouse 1-cell stage. The nuclear-localization of ZAG1 protein in mouse preimplantation embryos at 15 hpi was confirmed by both subcellular analysis of enhanced green fluorescent protein (EGFP)-tagged ZAG1 and immunocytochemical analysis with anti-ZAG1 antibody. Subsequently, using yeast two-hybrid screening, we identified U2 small nuclear ribonucleoprotein B (U2B"), which is associated with pre-mRNA splicing, as a putative interacting partner of ZAG1 protein. Furthermore, knockdown of <i>zag1</i> expression by an antisense DNA plasmid induced arrest and/or delay of embryonic development in injected 1-cell embryos. These results suggest that ZAG1 may be closely associated with zygotic gene expression in mouse preimplantation embryos.<br>

収録刊行物

  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(3), 192-197, 2008-06-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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各種コード

  • NII論文ID(NAID)
    10021219342
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168818
  • NDL 記事登録ID
    9543024
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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