Conditioning of Karyoplasts for Producing Somatic Nuclear Transferred Gonadal Germ Cells in Domestic Chickens

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The objective of this study was to establish a protocol for generating karyoplasts that can be used to produce somatic nuclear transferred gonadal germ cells (snt-GGCs) in domestic chickens. Karyoplasts were produced by centrifuging cultured fibroblasts from 10-day-old chick embryos at 10,000 × <i>g</i> in the presence of 1.0 μg/ml cytochalasin B. The number of karyoplasts was significantly (P<0.05) higher and the diameters of the karyoplasts were significantly (P<0.05) smaller when fibroblasts were centrifuged for 60 min than for 10 or 30 min. It was possible to generate snt-GGCs by electrofusion of GGCs with karyoplasts produced from cryopreserved or serum-starved fibroblasts. These results indicate that karyoplasts generated from 10-day-old chick embryos can be used to produce snt-GGCs even after cryopreservation and serum starvation of the fibroblasts.<br>

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  • The Journal of reproduction and development  

    The Journal of reproduction and development 54(3), 221-224, 2008-06-01 

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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各種コード

  • NII論文ID(NAID)
    10021219510
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168818
  • NDL 記事登録ID
    9543133
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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