Effects of Aromatase Inhibitor (Fadrozole)-Induced Sex-Reversal on Gonadal Differentiation and mRNA Expression of P450arom, AMH and ERα in Embryos and Growth in Posthatching Quail

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著者

    • Koba Naomi KOBA Naomi
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • MIZUSHIMA Shusei
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • TSUKADA Akira
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • SAITO Noboru
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • ONO Tamao
    • Department of Food Production Science, Faculty of Agriculture, Shinshu University
    • SHIMADA Kiyoshi
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University

抄録

The present study was conducted to investigate (1) the effective day of treatment with Fadrozole, nonsteroidal aromatase inhibitor (AI), during incubation for sex reversal, (2) the mRNA expression of aromatase (P450arom), anti-Müllerian hormone (AMH) and estrogen receptor α (ERα) in quail gonad and gonadal structures after the AI treatment before hatching and (3) the effects of AI on gonad growth, left-right asymmetry of the gonad and body weight gain between 1 to 7 weeks posthatch. (1) Females AI-treated at 0, 2 and 4 days of incubation had symmetrical gonads at hatch similar to normal males, whereas those treated at 6 and 8 days of incubation had asymmetrical gonads. (2) In females AI-treated at day 0 of incubation, the left gonad became an ovotestis at day 15 of incubation, displaying seminiferous tubules with testicular-like cords and ovarian follicle with ovum. In the control group, P450arom mRNA expression was significantly higher in females than males. However, AI-treated females showed 2 response-patterns of either higher or lower expression. The latter was similar to that of control males. No significant differences in levels of AMH and ERα mRNA between sexes either in control or AI-treated groups was observed. (3) A gradual increase in body weight of the control males and females without sex difference was observed up to 5 weeks of age. However, at 6-7 weeks the average weight of females was heavier than males each in control and treated group. These results indicate that shortly before day 6 of incubation P450arom mRNA expression, at least in a part, is important for asymmetrical formation of gonads in female quail. In AI-treated females, the high responder show similar level of P450arom mRNA in control males and the low responder do not respond to the AI. Body weight is controlled non-gonadally in quail.

収録刊行物

  • The journal of poultry science

    The journal of poultry science 45(2), 116-124, 2008-04-25

    日本家禽学会

参考文献:  38件中 1-38件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10021220131
  • NII書誌ID(NCID)
    AA11564513
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    13467395
  • NDL 記事登録ID
    9469222
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-J694
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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