Profiles of mRNA Expression of FOXL2, P450arom, DMRT1, AMH, P450_<c17>, SF1, ERα and AR, in Relation to Gonadal Sex Differentiation in Duck Embryo

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著者

    • Koba Naomi KOBA Naomi
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • MIZUSHIMA Shusei
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • TSUKADA Akira
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • SAITO Noboru
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
    • SHIMADA Kiyoshi
    • Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University

抄録

The present study was conducted to show a profile of mRNA expression of sex-differentiation related genes in the duck gonad. Fertile duck eggs (Cherry-valley) were incubated (hatching at day 28 of incubation) and the gonads were collected at days 7, 8, 10, 12 and 15 of incubation after taking photos of the gonads. Left gonad was used for identification of genetic sex and for total RNA extraction. Reverse transcription-polymerase chain reaction (PCR) was performed using appropriate forward and reverse primers for each of FOXL2, P450arom, DMRT1, AMH, P450<SUB>c17</SUB>, SF1, ER α and AR genes of the chicken and the corresponding cDNA fragments were sequenced for duck. Subsequently, real-time PCR was performed to detect mRNA expression of those genes in the duck embryo. Male bilateral gonads assumed symmetry in appearance throughout the examined period, whereas the female gonads were noticeably asymmetric at 10 days of incubation with a smaller size of the right. Female asymmetry was distinct by day 15 of incubation. In females, FOXL2 and P450arom mRNA expression began at day 8 of incubation and remained high thereafter, but in males only negligible expression was detected. In males, mRNA expression of DMRT1 and AMH was detected at day 7 of incubation and tended to increase gradually thereafter, whereas in females the expression remained low throughout the examined period. Though the slight expression of P450<SUB>c17</SUB> mRNA started at day 7 only in males, the expression was low throughout the examined period, whereas in female the expression was distinct at day 8 of incubation and remained high thereafter. Expressions of the other genes were variable with no differences in each expression between the sexes. The results indicate that FOXL2 and P450arom play an important role for the ovarian formation.

収録刊行物

  • The journal of poultry science  

    The journal of poultry science 45(2), 132-138, 2008-04-25 

    日本家禽学会

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キーワード

各種コード

  • NII論文ID(NAID)
    10021220193
  • NII書誌ID(NCID)
    AA11564513
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13467395
  • NDL 記事登録ID
    9469250
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-J694
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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