An Ultimate Method for Detection of Infected Pathogenic Microorganism from silkworm using HDGP

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  • Hatakeyama Yoshinori
    Laboratory of applied entomology, Department of plant science and resources, College of bioresource sciences, Nihon University
  • Hamano Keiichi
    TAITEC Corporation
  • Iwano Hidetoshi
    Laboratory of applied entomology, Department of plant science and resources, College of bioresource sciences, Nihon University

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For detection and identification of pathogenic microorganisms which were infected with silkworm, Bombyx mori using PCR, amplification of DNA of generally assumed pathogenic microorganisms has been performed using specific primers. Thus, detection of unknown pathogens with the possibility to infect silkworms requires designing new specific primers, which takes time before detection. To develop a method of detecting silkworm infected with all pathogenic microorganisms by an identical procedure under identical conditions, we designed Hyper Detection of infectious disease based on Genome Profiling (HDGP). HDGP is a new technique utilizing the concept of genome profiling (GP), and it is capable of simultaneously detecting infected silkworm and identifying the cause of infection without specific primers. We detected entomopathogenic protozoa that infected silkworm and virus DNA-contaminated silkworms DNA to investigate the usefulness of HDGP, and confirmed significant differences in the profile image between the pathogenic microorganisms-contaminated silkworm and healthy silkworm. We also obtained similar results in a comparative experiment of assumptive infected tomatoes and pathogenic fungus. These findings suggest that HDGP may detect infections regardless of the species of host and infectious pathogen.<br>

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