An Ultimate Method for Detection of Infected Pathogenic Microorganism from Silkworm using HDGP

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For detection and identification of pathogenic microorganisms which were infected with silkworm, Bombyx mori using PCR, amplification of DNA of generally assumed pathogenic microorganisms has been performed using specific primers. Thus, detection of unknown pathogens with the possibility to infect silkworms requires designing new specific primers, which takes time before detection. To develop a method of detecting silkworm infected with all pathogenic microorganisms by an identical procedure under identical conditions, we designed Hyper Detection of infectious disease based on Genome Profiling (HDGP). HDGP is a new technique utilizing the concept of genome profiling (GP), and it is capable of simultaneously detecting infected silkworm and identifying the cause of infection without specific primers. We detected entomopathogenic protozoa that infected silkworm and virus DNA-contaminated silkworms DNA to investigate the usefulness of HDGP, and confirmed significant differences in the profile image between the pathogenic microorganisms-contaminated silkworm and healthy silkworm. We also obtained similar results in a comparative experiment of assumptive infected tomatoes and pathogenic fungus. These findings suggest that HDGP may detect infections regardless of the species of host and infectious pathogen.<br>

収録刊行物

  • Journal of insect biotechnology and sericology  

    Journal of insect biotechnology and sericology 77(1), 1-7, 2008-02-28 

    The Japanese Society of Sericultural Science

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各種コード

  • NII論文ID(NAID)
    10021220832
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    9400300
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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