Application of Real Time PCR for the Quantitative Detection of Radiation-induced Genomic DNA Strand Breaks

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抄録

The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with <sup>137</sup>Cs γ rays. We verified that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by γ ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.<br>

収録刊行物

  • Journal of insect biotechnology and sericology  

    Journal of insect biotechnology and sericology 77(1), 17-24, 2008-02-28 

    The Japanese Society of Sericultural Science

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各種コード

  • NII論文ID(NAID)
    10021220872
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    9400339
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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