Purification and cDNA Cloning of Vitellogenin of the Wild Silkworm, Saturnia japonica (Lepidoptera : Saturniidae)

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著者

    • Meng Yan MENG Yan
    • Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu University
    • NAKAGAKI Masao
    • Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu University
    • KAJIURA Zenta
    • Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu University

抄録

We purified the major yolk protein, vitellin, from Saturnia japonica, by column chromatographies. SDS-PAGE and immunoblot analysis of the S. japonica vitellin (SjVn) showed that SjVn consisted of only a large subunit with a molecular size of approximately 200kDa. We then cloned and sequenced cDNA of the S. japonica vitellogenin (SjVg), a SjVn precursor. The SjVg cDNA was 5731 nucleotides long and encoded 1776 amino acids for the entire subunit. The molecular weight of the predicted polypeptide was 200,000. Consensus motifs, such as GL/ICG (at the amino acid position 1592) and DGGR (located 17 residues upstream from the GL/ICG motif) were found in the deduced amino acid sequence. There is no RXRR motif, which is a cleavage site between the small and large subunits. Two polyserine regions were found in the deduced amino acid sequence.<br>

収録刊行物

  • Journal of insect biotechnology and sericology  

    Journal of insect biotechnology and sericology 77(1), 35-44, 2008-02-28 

    The Japanese Society of Sericultural Science

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各種コード

  • NII論文ID(NAID)
    10021220932
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    9400389
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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