Mapping of a Novel Virus Resistant Gene, Nid-1, in the Silkworm, Bombyx mori, Based on the Restriction Fragment Length Polymorphism (RFLP)

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In the silkworm, non-susceptibility to B. mori densonucleosis virus type-1 (DNV-1) is controlled by a dominant gene, Nid-1. In this report, simple and effective methods for linkage analysis and for mapping were improved. Three strains were used: No. 908, a virus resistant strain carrying Nid-1, and RF02 and RF50, which were used as standard wild type strains in our previous linkage analyses and mapping. Linkage analysis of Nid-1 was carried out by using survivors of two kinds of backcrosses, (RF50×908) females crossed to RF02 males and (RF02 × 908) females crossed to an RF50 male, after oral inoculation with DNV-1. The ordered DNA patterns from two families of BF<sub>1</sub> segregants was enough to determine linkage group by analysis of RFLP patterns of previous mapped cDNA clones, specific for each of the twenty-eight linkage groups (RFLGs). The results indicated that Nid-1 was linked to linkage group seventeen (RFLG17). Individual genomic DNA samples of 64 survivors from the backcross of RF02×(RF50×908) were used to locate five clones and Nid-1. The relative distances between them were determined by repeated three-point analysis and showed that Nid-1 was closely linked to two genes (m025 and m237), at a distance of 4.7cM from them.<br>

収録刊行物

  • Journal of insect biotechnology and sericology  

    Journal of insect biotechnology and sericology 77(1), 59-66, 2008-02-28 

    The Japanese Society of Sericultural Science

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各種コード

  • NII論文ID(NAID)
    10021221024
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    9400454
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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