Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

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著者

    • Banno Yutaka BANNO Yutaka
    • Institute of Genetic Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
    • NHO Si Kab
    • College of Agriculture and Life Sciences, Kyungpook National University
    • TU Zhenli
    • College of Animal Sciences, Zhejiang University
    • MITA Kazuei
    • Division of Insect Science, National Institute of Agrobiological Sciences
    • TSUCHIDA Kozo
    • Division of Radiological Protection and Biology, National Institute of Infectious Diseases
    • TAKADA Naoko
    • Division of Radiological Protection and Biology, National Institute of Infectious Diseases
    • MAEKAWA Hideaki
    • Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus
    • NAKAJIMA Yumiko
    • Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus

抄録

The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan)(Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.<br>

収録刊行物

  • Journal of insect biotechnology and sericology  

    Journal of insect biotechnology and sericology 77(2), 79-85, 2008-06-30 

    The Japanese Society of Sericultural Science

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各種コード

  • NII論文ID(NAID)
    10021221089
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    9573995
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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