Method for rapid distinction of <i>Bombyx mandarina</i> (Japan) from <i>B. mandarina</i> (China) based on rDNA sequence differences

• Kawanishi Yuichi

  Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus

• Banno Yutaka

  Institute of Genetic Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Fujimoto Hirofumi

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Kab Nho Si

  College of Agriculture and Life Sciences, Kyungpook National University

• Tu Zhenli

  College of Animal Sciences, Zhejiang University

• Mita Kazuei

  Division of Insect Science, National Institute of Agrobiological Sciences

• Tsuchida Kozo

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Takada Naoko

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Maekawa Hideaki

  Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Nakajima Yumiko

  Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus

Bibliographic Information

Other Title

• Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

Abstract

The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan)(Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.<br>

Journal

• Journal of Insect Biotechnology and Sericology

  Journal of Insect Biotechnology and Sericology 77 (2), 2_79-2_85, 2008

  The Japanese Society of Sericultural Science

Keywords

• rDNA
• B. mandarina
• B. mori
• karyotype
• chromosome #11
• PCR

Details

• CRID: 1390001205197208960
• NII Article ID: 10021221089
• NII Book ID: AA11558849
• DOI: 10.11416/jibs.77.2_79
• ISSN: 18847978
• ISSN: 13468073
• NDL BIB ID: 9573995
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9573995
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed